Proteintech

MARCH8 Polyclonal Antibody

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MARCH8 Antibody (14119-1-AP) in WB

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14119-1-AP (MARCH8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

MARCH8 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14119-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MARCH8 Fusion Protein Ag5268 (1-158 aa encoded by BC066988)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSMPLHQIS AIPSQDAISA RVYRSKTKEK EREEQNEKTL GHFMSHSSNI SKAGSPPSAS APAPVSSFSR TSITPSSQDI CRICHCEGDD ESPLITPCHC TGSLHFVHQA CLQQWIKSSD TRCCELCKYE FIMETKLKPL RKWEKLQMTS SERRKIMCS (1-158 aa encoded by BC066988 )

Target Information

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N protein of PEDV plays chess game with host proteins by selective autophagy.

Autophagy

Zhai X,Kong N,Zhang Y,Song Y,Qin W,Yang X,Ye C,Ye M,Tong W,Liu C,Zheng H,Yu H,Zhang W,Yang X,Zhang G,Tong G,Shan T

Tue Aug 01 00:00:00 EDT 2023

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα.

Signal transduction and targeted therapy

Feng L,Li C,Zeng LW,Gao D,Sun YH,Zhong L,Lin H,Shu HB,Li S

Mon Jan 24 00:00:00 EST 2022

TARDBP Inhibits Porcine Epidemic Diarrhea Virus Replication through Degrading Viral Nucleocapsid Protein and Activating Type I Interferon Signaling.

Journal of virology

Dong S,Kong N,Zhang Y,Li Y,Sun D,Qin W,Zhai H,Zhai X,Yang X,Ye C,Ye M,Liu C,Yu L,Zheng H,Tong W,Yu H,Zhang W,Tong G,Shan T

Wed May 25 00:00:00 EDT 2022

FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.

Journal of virology

Dong S,Kong N,Wang C,Li Y,Sun D,Qin W,Zhai H,Zhai X,Yang X,Ye C,Ye M,Liu C,Yu L,Zheng H,Tong W,Yu H,Zhang W,Tong G,Shan T

Wed Jul 13 00:00:00 EDT 2022

2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.

Autophagy

Sun D,Kong N,Dong S,Chen X,Qin W,Wang H,Jiao Y,Zhai H,Li L,Gao F,Yu L,Zheng H,Tong W,Yu H,Zhang W,Tong G,Shan T

Mon Aug 01 00:00:00 EDT 2022

Elucidating the Antiviral Mechanism of Different MARCH Factors.

mBio

Umthong S,Lynch B,Timilsina U,Waxman B,Ivey EB,Stavrou S

Tue Mar 02 00:00:00 EST 2021

EGR1 Suppresses Porcine Epidemic Diarrhea Virus Replication by Regulating IRAV To Degrade Viral Nucleocapsid Protein.

Journal of virology

Wang H,Kong N,Jiao Y,Dong S,Sun D,Chen X,Zheng H,Tong W,Yu H,Yu L,Zhang W,Tong G,Shan T

Thu Sep 09 00:00:00 EDT 2021

Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.

Molecular cancer research : MCR

Qian G,Guo J,Vallega KA,Hu C,Chen Z,Deng Y,Wang Q,Fan S,Ramalingam SS,Owonikoko TK,Wei W,Sun SY

Fri Oct 01 00:00:00 EDT 2021

MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.

Cancers

Chen W,Patel D,Jia Y,Yu Z,Liu X,Shi H,Liu H

Sat May 22 00:00:00 EDT 2021

The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction.

Human gene therapy

Xu F,Liu X,Zhang D,Zhao F,Fan Z,Hu S,Mei S,Huang Y,Sun H,Wei L,Guo L,Wang J,Cen S,Liang C,Guo F

Wed Sep 01 00:00:00 EDT 2021

The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction.

Human gene therapy

Xu F,Liu X,Zhang D,Zhao F,Fan Z,Hu S,Mei S,Huang Y,Sun H,Wei L,Guo L,Wang J,Cen S,Liang C,Guo F

Wed Sep 01 00:00:00 EDT 2021

PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective Autophagy.

Microbiology spectrum

Jiao Y,Kong N,Wang H,Sun D,Dong S,Chen X,Zheng H,Tong W,Yu H,Yu L,Huang Y,Wang H,Sui B,Zhao L,Liao Y,Zhang W,Tong G,Shan T

Sun Oct 31 00:00:00 EDT 2021

MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.

Nature communications

Liu X,Xu F,Ren L,Zhao F,Huang Y,Wei L,Wang Y,Wang C,Fan Z,Mei S,Song J,Zhao Z,Cen S,Liang C,Wang J,Guo F

Tue Jul 20 00:00:00 EDT 2021

MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation.

mBio

Yu C,Li S,Zhang X,Khan I,Ahmad I,Zhou Y,Li S,Shi J,Wang Y,Zheng YH

Tue Sep 15 00:00:00 EDT 2020

BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.

Autophagy

Kong N,Shan T,Wang H,Jiao Y,Zuo Y,Li L,Tong W,Yu L,Jiang Y,Zhou Y,Li G,Gao F,Yu H,Zheng H,Tong G

Thu Oct 01 00:00:00 EDT 2020

Tetherin Suppresses Type I Interferon Signaling by Targeting MAVS for NDP52-Mediated Selective Autophagic Degradation in Human Cells.

Molecular cell

Jin S,Tian S,Luo M,Xie W,Liu T,Duan T,Wu Y,Cui J

Thu Oct 19 00:00:00 EDT 2017

Tetherin Suppresses Type I Interferon Signaling by Targeting MAVS for NDP52-Mediated Selective Autophagic Degradation in Human Cells.

Molecular cell

Jin S,Tian S,Luo M,Xie W,Liu T,Duan T,Wu Y,Cui J

Thu Oct 19 00:00:00 EDT 2017

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Oncogenesis

Wang Q,Chen Q,Zhu L,Chen M,Xu W,Panday S,Wang Z,Li A,Røe OD,Chen R,Wang S,Zhang R,Zhou J

Mon Jul 03 00:00:00 EDT 2017

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Oncogenesis

Wang Q,Chen Q,Zhu L,Chen M,Xu W,Panday S,Wang Z,Li A,Røe OD,Chen R,Wang S,Zhang R,Zhou J

Mon Jul 03 00:00:00 EDT 2017

Cell adhesion in zebrafish embryos is modulated by March 8.

PloS one

Kim MH,Rebbert ML,Ro H,Won M,Dawid IB

Tue Jan 20 00:00:00 EST 2015

Cell adhesion in zebrafish embryos is modulated by March 8.

PloS one

Kim MH,Rebbert ML,Ro H,Won M,Dawid IB

Tue Jan 20 00:00:00 EST 2015

The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.

Proceedings of the National Academy of Sciences of the United States of America

Chen R,Li M,Zhang Y,Zhou Q,Shu HB

Tue Aug 28 00:00:00 EDT 2012

The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.

Proceedings of the National Academy of Sciences of the United States of America

Chen R,Li M,Zhang Y,Zhou Q,Shu HB

Tue Aug 28 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: c-MIR; c-mir, cellular modulator of immune recognition; Cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR); E3 ubiquitin-protein ligase MARCHF8; MARCH-VIII; membrane associated ring finger 8; membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCHF8; RP11-67C2.1

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Gene Aliases: 1300017E09Rik; c-MIR; CMIR; MARCH-VIII; MARCH8; MARCHF8; MIR; RNF178

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UniProt ID: (Human) Q5T0T0, (Mouse) Q9DBD2

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Entrez Gene ID: (Human) 220972, (Mouse) 71779

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Function(s)

ubiquitin-protein transferase activity zinc ion binding ligase activity MHC class II protein binding ubiquitin protein ligase activity

Process(es)

protein polyubiquitination adaptive immune response antigen processing and presentation of peptide antigen via MHC class II negative regulation of MHC class II biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MARCH8 Polyclonal Antibody

MARCH8 Antibody (14119-1-AP) in WB

Product Details

14119-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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