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Application
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MAST4 Antibody (PA5-51407) in ICC/IF
Immunofluorecent analysis of MAST4 in human cell line A-431 using MAST4 Polyclonal Antibody (Product # PA5-51407). Staining shows localization to nucleoplasm and cytosol.
Product Details
PA5-51407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MAST4. Recombinant protein control fragment (Product #RP-93710).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2643777
Product Specific Information
Immunogen sequence: PEKQESHQKS HGPGSDLENF ALFKLEEREK KVYPKAVERS STFENKASMQ EAPPLGSLLK DALHKQASVR ASEGAMSDGP VPAEHRQGGG DFRRAPAPGT LQDGLCHS
Highest antigen sequence identity to the following orthologs: Mouse - 60%, Rat - 60%.
Target Information
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine(Ser/Thr) protein kinases. MAST4 (microtubule associated serine/threonine kinase family member 4), also known as KIAA0303, is a 2,626 amino acid protein that localizes to the cytoplasm and contains one protein kinase domain, as well as one PDZ domain and one AGC-kinase C-terminal domain. Expressed at high levels in most adult tissues, including testis, colon and small intestine, MAST4 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Multiple isoforms of MAST4 exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Microtubule-associated serine/threonine-protein kinase 4
Gene Aliases: KIAA0303; MAST4
UniProt ID: (Human) O15021
Entrez Gene ID: (Human) 375449
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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