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Invitrogen

MCM2 Monoclonal Antibody (2B3)

Advanced Verification

3 Published Figures

5 References

View all (76) MCM2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

MCM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

MCM2 Antibody (MA5-15895) in IHC (P)

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 monoclonal antibody (Product # MA5-15895) followed with DAB staining.

MCM2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

MCM2 Antibody — Figure 7 PHA-767491 modulates TCR signaling. (A) PHA-767491 treatment suppresses Erk phosphorylation. Immunoblots of OT-I CTL that were not-treated (-) or were treated (+) with PHA-767491 were stimulated with K b -OVA tetramers for the indicated times. Normalized values of individual bands are indicated below the respective bands. Representative blots of at least three independent experiments are shown. (B) The ratio of the band intensities of the phosphorylated proteins to the respective total proteins from (A) are shown in the bar charts, depicted as mean +- SEM. Values represent fold change, normalized to the DMSO-treated sample values for the respective time points. (C) PHA-767491 does not impair Ca 2+ flux. Peripheral lymphocytes were either pre-treated with PHA-767491 or not and were incubated with biotinylated anti-CD3 antibodies. Lymphocytes were first cross-linked using streptavidin, followed by an exogenous addition of CaCl 2 solution, and finally treated with ionomycin. (D) PHA-767491 affects TCR recycling in stimulation assays. Peripheral lymphocytes and thymocytes were stimulated with plate-bound anti-CD3 antibody or anti-CD3/CD28 beads, respectively, for 3 or 17 h. Values indicated in the histograms represent the MFI. (E) Cdc7 association with Dbf4 and MCM2 is reduced in TCR stimulated T cells treated with PHA-767491. Serum-starved Jurkat cells that were not-treated (-) or treated (+) with PHA-767491 were seeded into wells coated with plate-bound anti-CD3 antibo {TM}

Product Details

MA5-15895

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B3

Immunogen

Purified recombinant fragment of human MCM2 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11155194

Product Specific Information

MA5-15895 targets MCM2 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15895 immunogen is purified recombinant fragment of human MCM2 expressed in E. Coli. .

MA5-15895 detects MCM2 which has a predicted molecular weight of approximately 125kDa.

Target Information

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

Published figure using MCM2 monoclonal antibody (Product # MA5-15895) in Immunocytochemistry

Fri Mar 26 00:00:00 UTC 2021

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

Published figure using MCM2 monoclonal antibody (Product # MA5-15895) in Immunocytochemistry

Fri Mar 26 00:00:00 UTC 2021

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

Published figure using MCM2 monoclonal antibody (Product # MA5-15895) in Immunocytochemistry

Fri Mar 26 00:00:00 UTC 2021

Co-Administration of Fendiline Hydrochloride Enhances Chemotherapeutic Efficacy of Cisplatin in Neuroblastoma Treatment.

Molecules (Basel, Switzerland)

Brizzolara A,Garbati P,Vella S,Calderoni M,Quattrone A,Tonini GP,Capasso M,Longo L,Barbieri R,Florio T,Pagano A

MA5-15895 was used in Immunohistochemistry to suggest that fendiline hydrochloride can enhance cisplatin anti-tumour activity during neuroblastoma treatment.

Tue Nov 10 00:00:00 UTC 2020

A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation.

Frontiers in immunology

Chen EW,Tay NQ,Brzostek J,Gascoigne NRJ,Rybakin V

Published figure using MCM2 monoclonal antibody (Product # MA5-15895) in Western Blot

Tue Oct 13 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; MGC10606; mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

Gene Aliases: AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; Mcmd2; MITOTIN; mKIAA0030

UniProt ID: (Human) P49736, (Mouse) O08971

Entrez Gene ID: (Human) 4171, (Rat) 312538, (Mouse) 17216

Function(s)

DNA binding DNA helicase activity DNA replication origin binding protein binding ATP binding enzyme binding histone binding metal ion binding nucleotide binding helicase activity hydrolase activity DNA metabolism protein

Process(es)

G1/S transition of mitotic cell cycle DNA replication DNA unwinding involved in DNA replication DNA replication initiation nucleosome assembly cell cycle cellular response to interleukin-4

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MCM2 Monoclonal Antibody (2B3)

MCM2 Antibody (MA5-15895) in IHC (P)

Product Details

MA5-15895

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions