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Invitrogen
MCM5 Polyclonal Antibody
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Application
FIGURE: 1 / 4
MCM5 Antibody (PA5-29913) in ICC/IF
MCM5 Polyclonal Antibody detects MCM5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM5 protein stained by MCM5 Polyclonal Antibody (Product # PA5-29913) diluted at 1:500. Blue: Hoechst 33343 staining.
Product Details
PA5-29913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MCM5. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.41 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547387
Product Specific Information
Recommended positive controls: HeLa.
Predicted reactivity: Mouse (95%), Xenopus laevis (88%), Chicken (87%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46
Gene Aliases: CDC46; MCM5; P1-CDC46
UniProt ID: (Human) P33992
Entrez Gene ID: (Human) 4174
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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