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Invitrogen

ME1 Polyclonal Antibody

Advanced Verification

View all (17) ME1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (1)

Application

ME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

ME1 Antibody (PA5-82251) in IHC (P)

Immunohistochemical analysis of ME1 in human testis using ME1 Polyclonal Antibody (Product # PA5-82251) shows moderate cytoplasmic positivity in cells in seminiferous ducts.

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ME1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ME1 demonstrates antibody specificity. Immunohistochemical analysis of ME1 using anti-ME1 Polyclonal Antibody (Product #PA5-82251), shows significant staining of ME1 in adrenal gland and shows minimal or weak staining in kidney tissues. The relative expression levels of ME1 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82251

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ME1. Recombinant protein control fragment (Product #RP-88951).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2789411

Product Specific Information

Immunogen sequence: RITKNKLSDQ TILFQGAGEA ALGIAHLIVM ALEKEGLPKE KAIKKIWLVD SKGLIVKGRA SLTQEKEKFA HEHEEMKNLE AIVQEIKPTA LIGVAAIGGA FSEQILKDMA AFNERPIIFA LSNPTSKAEC

Target Information

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase

Gene Aliases: HUMNDME; ME1; MES

UniProt ID: (Human) P48163

Entrez Gene ID: (Human) 4199

Function(s)

malic enzyme activity malate dehydrogenase (decarboxylating) (NAD+) activity malate dehydrogenase (decarboxylating) (NADP+) activity oxaloacetate decarboxylase activity electron carrier activity manganese ion binding ADP binding NADP binding NAD binding oxidoreductase

Process(es)

carbohydrate metabolic process malate metabolic process NADP biosynthetic process response to hormone response to carbohydrate protein tetramerization oxidation-reduction process regulation of NADP metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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