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Invitrogen
MED10 Polyclonal Antibody
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Application
FIGURE: 1 / 2
MED10 Antibody (PA5-78599) in ICC/IF
MED10 Polyclonal Antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 Polyclonal Antibody (Product # PA5-78599) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-78599
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MED10. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.03 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736133
Product Specific Information
Positive Control: 293T, 293T nuclear extract
Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (85%), Xenopus laevis (92%), Rhesus Monkey (98%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MED10 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Mediator complex subunit 10; Mediator of RNA polymerase II transcription subunit 10; mediator of RNA polymerase II transcription, subunit 10; Transformation-related gene 17 protein; Transformation-related gene 20 protein; TRG-17; TRG-20
Gene Aliases: L6; MED10; NUT2; TRG17; TRG20
UniProt ID: (Human) Q9BTT4
Entrez Gene ID: (Human) 84246
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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