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Invitrogen
MED12 Polyclonal Antibody
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Application
FIGURE: 1 / 15
MED12 Antibody (PA5-51852) in ICC/IF
Immunofluorescence analysis of MED12 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MED12 Polyclonal Antibody (Product # PA5-51852) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
Product Details
PA5-51852
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MED12. Recombinant protein control fragment (Product #RP-92262).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2643843
Product Specific Information
Immunogen sequence: LPTTMTGVMG LEPSSYKTSV YRQQQPAVPQ GQRLRQQLQA KIQSQGMLGQ SSVHQMTPSS SYGLQTSQGY TPYVSHVGLQ QHTGPAGTMV PPSYSSQPYQ STHPSTNPTL VDPTRHLQQR PSGYVHQQAP TYGHGLTS
Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.
Target Information
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; human opposite paired; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OPA-containing protein; putative mediator subunit 12; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; Trap230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
Gene Aliases: ARC240; CAGH45; FGS1; HOPA; KIAA0192; MED12; MED12S; OHDOX; OKS; OPA1; TNRC11; TRAP230
UniProt ID: (Human) Q93074
Entrez Gene ID: (Human) 9968
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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