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Invitrogen
MED14 Polyclonal Antibody
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Application
FIGURE: 1 / 1
MED14 Antibody (PA5-87913) in WB
Western blot analysis of MED14 in extracts of various cell lines. Samples were incubated with MED14 Polyclonal antibody (Product # PA5-87913) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-87913
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_0042202)
Conjugate
Unconjugated
Form
Liquid
Concentration
3.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804499
Product Specific Information
Immunogen sequence: LDQATLDDME KSVNDDMKRI IPWIQQLKFW LGQQRCKQSI KHLPTISSET LQLSNYSTHP IGNLSKNKLF IKLTRLPQYY IVVEMLEVPN KPTQLSYKYY FMSVNAADRE DSPAMALLLQ QFKENIQDLV FRTKTGKQTR TNAKRKLSDD PCPVESKKTK RAGEMCAFNK VLAHFVAMCD T; Positive Samples: HeLa, SW480, Jurkat, Mouse brain, Mouse pancreas; Cellular Location: Nucleus
Target Information
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; cofactor required for Sp1 transcriptional activation subunit 2 (150 kDa); cofactor required for Sp1 transcriptional activation, subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP complex subunit 2; DRIP150; hRGR1; human homolog of yeast RGR1; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; MGC104513; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex component TRAP170; transcriptional co-activator CRSP150; Transcriptional coactivator CRSP150; Trap170; vitamin D receptor-interacting protein complex component DRIP150; Vitamin D3 receptor-interacting protein complex 150 kDa component
Gene Aliases: 9930001L01Rik; ARC150; AU041628; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; ENSMUSG00000073278; EXLM1; Gm641; MED14; ORF1; RGD1560170; RGR1; TRAP170
UniProt ID: (Human) O60244, (Mouse) A2ABV5
Entrez Gene ID: (Human) 9282, (Mouse) 26896, (Rat) 317343
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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