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MEK1 Antibody (BS-1433R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes ... View More
Product Details
BS-1433R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human MEK1, amino acids 2-150.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1
UniProt ID: (Human) Q02750, (Mouse) P31938, (Rat) Q01986
Entrez Gene ID: (Human) 5604, (Mouse) 26395, (Rat) 170851
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
receptor signaling protein tyrosine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
protein kinase binding
protein serine/threonine kinase activator activity
protein N-terminus binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Ras GTPase binding
mitogen-activated protein kinase kinase kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
movement of cell or subcellular component
chemotaxis
cell cycle arrest
signal transduction
heart development
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
cerebellar cortex formation
neuron differentiation
keratinocyte differentiation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
cell motility
positive regulation of axonogenesis
Bergmann glial cell differentiation
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
placenta blood vessel development
labyrinthine layer development
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
cellular senescence
mitophagy in response to mitochondrial depolarization
positive regulation of production of miRNAs involved in gene silencing by miRNA
regulation of early endosome to late endosome transport
regulation of protein phosphorylation
regulation of vascular smooth muscle contraction
protein phosphorylation
response to oxidative stress
mitotic nuclear division
cell proliferation
phosphorylation
positive regulation of cell migration
melanosome transport
positive regulation of transcription elongation from RNA polymerase II promoter
negative regulation of homotypic cell-cell adhesion
peptidyl-tyrosine dephosphorylation
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of Ras protein signal transduction
vesicle transport along microtubule
Golgi inheritance
response to axon injury
neuron projection morphogenesis
protein heterooligomerization
response to glucocorticoid
lung morphogenesis
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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