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Invitrogen
MEK1 Monoclonal Antibody (3D9)
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Application
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MEK1 Antibody (13-3500) in WB
Knockout of MEK1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR934388_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of MEK1 was performed by loading 30 µg of HeLa Cas9 (Lane 1), andHeLa MEK1 KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting Syst... View More
Product Details
13-3500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3D9
Immunogen
Purified MEK from human T cells and recombinant MEK1
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533012
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Related Products for
Western Blot
Bioinformatics
Protein Aliases: dual specificity mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase or Erk Kinase, Dual specificity mitogen-activated protein kinase kinase, involved in ras mediated vulval induction, LEThal LET-537 (42.8 kD) (mek-2); MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MEK1; MEK2; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; MAP2K1; MAPKK1; mek-2; MEK1; MEKK1; MKK1; PRKMK1; XELAEV_18018447mg
UniProt ID: (Human) Q02750, (Mouse) P31938, (Rat) Q01986, (Xenopus) Q05116
Entrez Gene ID: (Dog) 478347, (Human) 5604, (Mouse) 26395, (Rat) 170851, (Xenopus) 379991
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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