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Invitrogen
MERS Coronavirus Spike Protein S1 Polyclonal Antibody
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Application
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MERS Coronavirus Spike Protein S1 Antibody (PA5-119581) in WB
Western blot analysis of MERS Coronavirus Spike Protein S1 in non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg). Samples were incubated with MERS Coronavirus Spike Protein S1 polyclonal antibody (Product # PA5-119581) using a dilution of 1:5,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 5% SDS-PAGE.
Product Details
PA5-119581
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of MERS-CoV Spike (S1) (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.73 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913154
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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