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Application
FIGURE: 1 / 3
MGST1 Antibody (MA5-57077) in ICC/IF
Immunocytochemical staining of MGST1 in HepG2 cells using MGST1 Recombinant Monoclonal Antibody (Product # MA5-57077) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Microsomal glutathione S-transferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-57077
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB5110
Immunogen
A synthesized peptide derived from human MGST1 (aa 20-55)
Conjugate
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
Target Information
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutathione S-transferase 12; MGC14525; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I
Gene Aliases: 1500002K10Rik; Gst; GST12; MGST; MGST-I; MGST1
UniProt ID: (Human) P10620, (Mouse) Q91VS7
Entrez Gene ID: (Human) 4257, (Mouse) 56615
glutathione metabolic process
xenobiotic metabolic process
response to organonitrogen compound
response to lipopolysaccharide
Leydig cell differentiation
response to drug
oxidation-reduction process
protein homotrimerization
cellular response to lipid hydroperoxide
cellular oxidant detoxification
glutathione derivative biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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