Invitrogen

MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), FITC, eBioscience™

12 Published Figures

17 References

View all (19) MHC Class I (H-2Kd/H-2Dd) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (12)

MHC Class I (H-2Kd/H-2Dd) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 13

MHC Class I (H-2Kd/H-2Dd) Antibody (11-5998-82) in Flow

Staining of BALB/c splenocytes with 0.5 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (blue histogram) or 0.5 µg of Anti-Mouse MHC Class I (H-2Kd/H-2Dd) FITC (purple histogram). Total viable cells were used for analysis.

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Product Details

11-5998-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Non-human primate

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

34-1-2S

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465358

Product Specific Information

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p.

Applications Reported: 34-1-2S has been reported for use in flow cytometric analysis.

Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Pharmacologic inhibition of IL11/STAT3 signaling increases MHC-I expression and T cell infiltration.

Journal of translational medicine

Xiong W,Chen Y,Zhang C,Li J,Huang H,Zhu Y,Deng G,Cheng J,Lin Y,Shi Z,Mou T

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Mon Jun 26 00:00:00 EDT 2023

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

PTCy ameliorates GVHD by restoring regulatory and effector T-cell homeostasis in recipients with PD-1 blockade.

Blood advances

Ikegawa S,Meguri Y,Kondo T,Sugiura H,Sando Y,Nakamura M,Iwamoto M,Maeda Y,Matsuoka KI

11-5998-82 was used in Flow Cytometry to present that the influence of PD-1 blockade differed within T-cell subsets and caused unbalanced reconstitution of T-cell subsets, resulting in severe GVHD. PTCy successfully restored T-cell homeostasis and ameliorated GVHD induced by PD-1-/- donor T cells.

Tue Dec 10 00:00:00 EST 2019

Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer.

Scientific reports

Nakazawa Y,Taniyama Y,Sanada F,Morishita R,Nakamori S,Morimoto K,Yeung KT,Yang J

11-5998 was used in Flow cytometry/Cell sorting to indicate that periostin's importance in EMT-dependent chemoresistance and is a promising target to overcome chemoresistance in triple-negative breast cancer.

Mon Mar 05 00:00:00 EST 2018

Genetic and phenotypic characterization of the novel mouse substrain C57BL/6N Korl with increased body weight.

Scientific reports

Choi KM,Jung J,Cho YM,Kim K,Kim MG,Kim J,Kim H,Shin HJ,Kim HD,Chung ST,Cho S,Chung MW

11-5998 was used in Immunocytochemistry-immunoflourescence to investigate the underlying genetic profiles of a newly developed mouse strain, C57BL/6NKorl.

Fri Oct 27 00:00:00 EDT 2017

Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification.

Journal of ovarian research

Zhang H,Yang Y,Wang Y,Gao X,Wang W,Liu H,He H,Liang Y,Pan K,Wu H,Shi J,Xue H,Liang L,Cai Z,Fan Y,Zhang Y

11-5998-82 was used in immunohistochemistry (frozen) to study the prospective association between CA125 and tumorigenic ovarian cancer cells, showing that CA125+ cells could form new tumours and may be ovarian cancer stem cells.

Sat Mar 28 00:00:00 EDT 2015

Model of fibrolamellar hepatocellular carcinomas reveals striking enrichment in cancer stem cells.

Nature communications

Oikawa T,Wauthier E,Dinh TA,Selitsky SR,Reyna-Neyra A,Carpino G,Levine R,Cardinale V,Klimstra D,Gaudio E,Alvaro D,Carrasco N,Sethupathy P,Reid LM

11-5998 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying hFL-HCCs pathogenesis and potential therapies.

Tue Oct 06 00:00:00 EDT 2015

Immunogenicity of undifferentiated and differentiated allogeneic mouse mesenchymal stem cells.

Journal of tissue engineering

Mukonoweshuro B,Brown CJ,Fisher J,Ingham E

11-5998 was used in Flow cytometry/Cell sorting to determine that although mesenchymal stem cells possess immunosuppressive properties, they may not be immunoprivileged.

Fri May 09 00:00:00 EDT 2014

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Thu May 28 00:00:00 EDT 2015

Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS one

Cheng CC,Lian WS,Hsiao FS,Liu IH,Lin SP,Lee YH,Chang CC,Xiao GY,Huang HY,Cheng CF,Cheng WT,Wu SC

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

The N terminus of adenovirus type 12 E1A inhibits major histocompatibility complex class I expression by preventing phosphorylation of NF-kappaB p65 Ser276 through direct binding.

Journal of virology

Jiao J,Guan H,Lippa AM,Ricciardi RP

11-5998 was used in Flow cytometry/Cell sorting to provide evidence for the role of the adenoviral E1A-12 N terminus as an NF-kappaB binding domain to inhibit the MHC I complex.

Sun Aug 01 00:00:00 EDT 2010

4-1BB functions as a survival factor in dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Choi BK,Kim YH,Kwon PM,Lee SC,Kang SW,Kim MS,Lee MJ,Kwon BS

11-5998 was used in Flow cytometry/Cell sorting to investigate the function of CD137 on dendritic cells, showing that it functions as a survival factor.

Wed Apr 01 00:00:00 EDT 2009

Tumorigenic adenovirus type 12 E1A inhibits phosphorylation of NF-kappaB by PKAc, causing loss of DNA binding and transactivation.

Journal of virology

Guan H,Jiao J,Ricciardi RP

11-5998 was used in Flow cytometry/Cell sorting to demonstrate that E1A-12 specifically prevents NF-kappaB from being phosphorylated by PKAc.

Tue Jan 01 00:00:00 EST 2008

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 11-5998-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2008

Evaluation of T-cell responses to peptides with MHC class I-binding motifs derived from PE_PGRS 33 protein of Mycobacterium tuberculosis.

Journal of medical microbiology

Chaitra MG,Shaila MS,Nayak R

11-5998 was used in Flow cytometry/Cell sorting to study how PE_PGRS 33 has access to the MHC class I processing pathway, and how short peptide fragments of this protein can be presented to CD8+ T cells.

Sun Apr 01 00:00:00 EDT 2007

Soluble type II transforming growth factor-beta receptor inhibits established murine malignant mesothelioma tumor growth by augmenting host antitumor immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Suzuki E,Kapoor V,Cheung HK,Ling LE,DeLong PA,Kaiser LR,Albelda SM

11-5998 was used in Flow cytometry/Cell sorting to suggest that TGF-beta blockade of established TGF-beta-secreting MM should be explored as a promising strategy to treat patients with tumors that produce TGF-beta.

Wed Sep 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D cell surface glycoprotein; H-2D(B); H-2K(B); H-2K(K); H-2K(Q); H2-DC1-beta transplantation antigen protein; H2DD; H2K1; H2KD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein

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Gene Aliases: H-2D; H-2K; H-2K(d); H2-D; H2-D1; H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01899, (Mouse) P01901

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Entrez Gene ID: (Mouse) 14964, (Mouse) 14972

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), FITC, eBioscience™

MHC Class I (H-2Kd/H-2Dd) Antibody (11-5998-82) in Flow

Product Details

11-5998-82

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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