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Invitrogen
MHC Class II I-Ad Monoclonal Antibody (AMS-32.1), APC, eBioscience™
FIGURE: 1 / 2
MHC Class II I-Ad Antibody (17-5323-82) in Flow
Staining of C57Bl/6 (left) and Balb/c (right) splenocytes with 0.125 µg of Mouse IgG2b k Isotype Control APC (Product # 17-4732-81) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class II I-Ad APC (purple histogram). Cells in the lymphocyte gate were used for analysis.
Product Details
17-5323-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AMS-32.1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10718531
Product Specific Information
Description: This AMS-32.1 monoclonal antibody reacts with the mouse MHC Class II I-Ad alloantigen, which is present on mouse strains such as Balb/c and DBA/2. This antibody also recognizes the H-2f, H-2g7, H-2i, and H-2v haplotypes. However, reactivity to other haplotypes (e.g., b, k, p, q, r, s, and u) has not been observed.
Applications Reported: This AMS-32.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AMS-32.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1
Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1
UniProt ID: (Mouse) P14483
Entrez Gene ID: (Mouse) 14961
B cell affinity maturation
immune system process
immunoglobulin production involved in immunoglobulin mediated immune response
humoral immune response mediated by circulating immunoglobulin
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
positive regulation of antigen processing and presentation
positive regulation of T-helper 1 type immune response
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of T cell proliferation
positive regulation of alpha-beta T cell activation
antigen processing and presentation of peptide antigen
cellular response to interferon-gamma
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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