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MLK4 Antibody (A302-610A) in WB
Detection of human MLK4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MLK4 antibody (Product # A302-610A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. MLK4 was also immunoprecipitated by rabbit anti-MLK4 antibody A302-609A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
产品信息
A302-610A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 986 and 1036 of human mixed lineage kinase 4.
偶联物
Unconjugated
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4 and does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mitogen-activated protein kinase kinase kinase 21; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK4
基因别名: dJ862P8.3; KIAA1804; MAP3K21; MLK4
UniProt ID: (Human) Q5TCX8
Entrez Gene ID: (Human) 84451
