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Application
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MMAE Antibody (MA5-50766) in ELISA
ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µL per well of MMAE-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4&(8)451;. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µL per well of MMAE monoclonal antibody (Product # MA5-50766) at concentration of 1 µg/mL with serial diluted MMAE starting from a concentration of 10 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 50 µL per well of an HRP... View More
Product Details
MA5-50766
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-04
Immunogen
MMAE-OVA
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3090452
Product Specific Information
Product MA5-50766 was used in a Competitive ELISA and Indirect ELISA.
Target Information
MMAE or monomethyl auristatin E, is a synthetic chemical compound that belongs to the class of tubulin inhibitors. It is commonly used as a payload in antibody-drug conjugates (ADCs) for targeted cancer therapy. MMAE functions by binding to tubulin, a protein involved in cell division, and disrupting the microtubule network necessary for cell division and growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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