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Invitrogen
MMAF Monoclonal Antibody (A5G12)
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Application
FIGURE: 1 / 2
MMAF Antibody (MA5-42537) in ELISA
ELISA analysis of MMAF Monoclonal Antibody (Product # MA5-42537) with MMAF-ADC, MMAF-BSA, MMAE-BSA and BSA protein at 1 μg/mL overnight at 4°C. Then blocked with 1% BSA for 1 hour at 37°C, and incubated with the primary antibody for 1 hour at 25°C. MMAF Monoclonal Antibody can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.
Product Details
MA5-42537
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A5G12
Immunogen
MMAF coupled with OVA
Conjugate
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911678
Product Specific Information
Product has been tested in direct ELISA
Target Information
Monomethyl auristatin F (MMAF) is a synthetic antineoplastic agent. It is part of the approved drug belantamab mafodotin in multiple myeloma and some experimental anti-cancer antibody-drug conjugates such as vorsetuzumab mafodotin and SGN-CD19A. In International Nonproprietary Names for MMAF-antibody-conjugates, the name mafodotin refers to MMAF plus its attachment structure to the antibody. Monomethyl auristatin F is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. It is linked to an antibody with high affinity to structures on cancer cells, causing MMAF to accumulate in such cells. MMAF is actually desmethyl-auristatin F; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin F itself.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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