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Application
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MRLC2 Antibody (PA5-78618) in WB
Western blot was performed using Anti-MRLC2 Rabbit Polyclonal Antibody (Product # PA5-78618) and a 20 kDa band corresponding to MRLC2 was observed in all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane1), Raji (Lane2), K-562 (Lane 3), HT-29 (Lane 4), RD (Lane 5), A549 (Lane 6), NIH-3T3 (Lane 7), HeLa (Lane 8), tissue extracts (30 µg lysate) of Mouse Heart (Lane 9) and Mouse Skeletal Muscle (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins we... View More
Product Details
PA5-78618
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRLC2. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736164
Product Specific Information
Positive Control: K562, Jurkat, Raji, NCI-H929
Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MRLC2 is involved in the the activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MLC-2; MLC-2A; MLC20; MLCB; MRLC3; myosin light chain, regulatory B; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; RLC; SHUJUN-1
Gene Aliases: 1500001M02Rik; C77744; MLC-B; MRLC2; MYL12B; MYLC2B; RLC-B
UniProt ID: (Human) O14950, (Mouse) Q3THE2
Entrez Gene ID: (Human) 103910, (Mouse) 67938
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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