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Application
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MRPL13 Antibody (PA5-96487) in IHC (P)
Immunohistochemistry analysis of MRPL13 in paraffin-embedded mouse kidney. Samples were incubated with MRPL13 Polyclonal antibody (Product # PA5-96487) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-96487
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human MRPL13 (NP_054797.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.85 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808289
Product Specific Information
Immunogen sequence: MSSFSRAPQQ WATFARIWYL LDGKMQPPGK LAAMASIRLQ GLHKPVYHAL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQV TAAQLHLRDP VAIVKLAIYG MLPKNLHRRT MMERLHLFPD EYIPEDILKN LVEELPQPRK IPKRLDEYTQ EEIDAFPRLW TPPEDYRL; Positive Samples: SH-SY5Y, HL-60, HT-29, A-549, HeLa, Mouse kidney, Mouse heart; Cellular Location: Mitochondrion
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 39S ribosomal protein L13, mitochondrial; L13mt; Large ribosomal subunit protein uL13m; MRP-L13
Gene Aliases: 1110002D09Rik; L13; L13A; L13mt; MRPL13; RPL13; RPML13
UniProt ID: (Human) Q9BYD1, (Mouse) Q9D1P0
Entrez Gene ID: (Human) 28998, (Mouse) 68537, (Rat) 299938
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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