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Proteintech

MRPL45 Polyclonal Antibody

Name: MRPL45 Polyclonal Antibody
Brand: Proteintech
SKU: 15682-1-AP
Price: 469.0 USD
Availability: InStock

12 References

View all (7) MRPL45 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)

Application

MRPL45 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

MRPL45 Antibody (15682-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 15682-1-AP (MRPL45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MRPL45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MRPL45 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15682-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MRPL45 Fusion Protein Ag8184 (86-329 aa encoded by BC006235)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AGLVIPPEK SDRSIHLACT AGIFDAYVPP EGDARISSLS KEGLIERTER MKKTMASQVS IRRIKDYDAN FKIKDFPEKA KDIFIEAHLC LNNSDHDRLH TLVTEHCFPD MTWDIKYKTV RWSFVESLEP SHVVQVRCSS MMNQGNVYGQ ITVRMHTRQT LAIYDRFGRL MYGQEDVPKD VLEYVVFEKQ LTNPYGSWRM HTKIVPPWAP PKQPILKTVM IPGPQLKPEE EYEEAQGEAQ KPQLA (86-329 aa encoded by B C006235)

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.

Human molecular genetics

Amarasekera SSC,Hock DH,Lake NJ,Calvo SE,Grønborg SW,Krzesinski EI,Amor DJ,Fahey MC,Simons C,Wibrand F,Mootha VK,Lek M,Lunke S,Stark Z,Østergaard E,Christodoulou J,Thorburn DR,Stroud DA,Compton AG

Thu Jul 20 00:00:00 UTC 2023

Nonstop mRNAs generate a ground state of mitochondrial gene expression noise.

Science advances

Ng KY,Lutfullahoglu Bal G,Richter U,Safronov O,Paulin L,Dunn CD,Paavilainen VO,Richer J,Newman WG,Taylor RW,Battersby BJ

Wed Nov 16 00:00:00 UTC 2022

Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.

The FEBS journal

Bruni F,Proctor-Kent Y,Lightowlers RN,Chrzanowska-Lightowlers ZM

Fri Jan 01 00:00:00 UTC 2021

An in vitro system to silence mitochondrial gene expression.

Cell

Cruz-Zaragoza LD,Dennerlein S,Linden A,Yousefi R,Lavdovskaia E,Aich A,Falk RR,Gomkale R,Schöndorf T,Bohnsack MT,Richter-Dennerlein R,Urlaub H,Rehling P

Thu Nov 11 00:00:00 UTC 2021

Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.

Journal of cell science

Lee RG,Gao J,Siira SJ,Shearwood AM,Ermer JA,Hofferek V,Mathews JC,Zheng M,Reid GE,Rackham O,Filipovska A

Fri Jul 24 00:00:00 UTC 2020

FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.

The Journal of cell biology

Wang L,Yan Z,Vihinen H,Eriksson O,Wang W,Soliymani R,Lu Y,Xue Y,Jokitalo E,Li J,Zhao H

Mon Jan 07 00:00:00 UTC 2019

FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.

The Journal of cell biology

Wang L,Yan Z,Vihinen H,Eriksson O,Wang W,Soliymani R,Lu Y,Xue Y,Jokitalo E,Li J,Zhao H

Mon Jan 07 00:00:00 UTC 2019

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Science advances

Rudler DL,Hughes LA,Perks KL,Richman TR,Kuznetsova I,Ermer JA,Abudulai LN,Shearwood AJ,Viola HM,Hool LC,Siira SJ,Rackham O,Filipovska A

Sun Dec 01 00:00:00 UTC 2019

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

RNA modification landscape of the human mitochondrial tRNA^Lys regulates protein synthesis.

Nature communications

Richter U,Evans ME,Clark WC,Marttinen P,Shoubridge EA,Suomalainen A,Wredenberg A,Wedell A,Pan T,Battersby BJ

Thu Sep 27 00:00:00 UTC 2018

RNA modification landscape of the human mitochondrial tRNA^Lys regulates protein synthesis.

Nature communications

Richter U,Evans ME,Clark WC,Marttinen P,Shoubridge EA,Suomalainen A,Wredenberg A,Wedell A,Pan T,Battersby BJ

Thu Sep 27 00:00:00 UTC 2018

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.

The Journal of biological chemistry

Zaganelli S,Rebelo-Guiomar P,Maundrell K,Rozanska A,Pierredon S,Powell CA,Jourdain AA,Hulo N,Lightowlers RN,Chrzanowska-Lightowlers ZM,Minczuk M,Martinou JC

Fri Mar 17 00:00:00 UTC 2017

Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.

Proceedings of the National Academy of Sciences of the United States of America

Akman G,Desai R,Bailey LJ,Yasukawa T,Dalla Rosa I,Durigon R,Holmes JB,Moss CF,Mennuni M,Houlden H,Crouch RJ,Hanna MG,Pitceathly RD,Spinazzola A,Holt IJ

Tue Jul 26 00:00:00 UTC 2016

Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.

Proceedings of the National Academy of Sciences of the United States of America

Akman G,Desai R,Bailey LJ,Yasukawa T,Dalla Rosa I,Durigon R,Holmes JB,Moss CF,Mennuni M,Houlden H,Crouch RJ,Hanna MG,Pitceathly RD,Spinazzola A,Holt IJ

Tue Jul 26 00:00:00 UTC 2016

MPV17L2 is required for ribosome assembly in mitochondria.

Nucleic acids research

Dalla Rosa I,Durigon R,Pearce SF,Rorbach J,Hirst EM,Vidoni S,Reyes A,Brea-Calvo G,Minczuk M,Woellhaf MW,Herrmann JM,Huynen MA,Holt IJ,Spinazzola A

Tue Jul 01 00:00:00 UTC 2014