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Invitrogen
MTHFD2 Polyclonal Antibody
Application
FIGURE: 1 / 9
MTHFD2 Antibody (PA5-28169) in ICC/IF
MTHFD2 Polyclonal Antibody detects MTHFD2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTHFD2 stained by MTHFD2 Polyclonal Antibody (Product # PA5-28169) diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-28169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 30 and 304 of Human MTHFD2
Conjugate
Unconjugated
Form
Liquid
Concentration
0.13 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545645
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, SK-N-DZ, SK-N-SH.
Predicted reactivity: Mouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
Gene Aliases: MTHFD2; NMDMC
UniProt ID: (Human) P13995
Entrez Gene ID: (Human) 10797
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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