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Invitrogen
MX1 Polyclonal Antibody
Application
FIGURE: 1 / 22
MX1 Antibody (PA5-22101) in ICC/IF
Immunofluorescence analysis of MX1 was performed using 70% confluent log phase HeLa and HeLa cells treated with IFN Alpha (10 ng/mL, 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MX1 Polyclonal Antibody (Product # PA5-22101) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More
Product Details
PA5-22101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Non-human primate,
Rabbit
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MX1. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.52 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153215
Product Specific Information
Recommended positive controls: Jurkat, Raji, NCI-H929, human MX1-transfected 293T.
Predicted reactivity: Rhesus Monkey (92%), Chimpanzee (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GTP-binding protein; IFI-78K; Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78; interferon-inducible myxovirus resistance-1 protein; interferon-inducible protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX dynamin-like GTPase 1; Myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; Myxovirus resistance protein 1
Gene Aliases: IFI-78K; IFI78; MX; MX1; Mx1-b; Mx1B; MxA
UniProt ID: (Human) P20591, (Bovine) P79135
Entrez Gene ID: (Human) 4599, (Bovine) 280872, (Rabbit) 100359014
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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