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Invitrogen
MYF5 Polyclonal Antibody
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Application
FIGURE: 1 / 2
MYF5 Antibody (PA5-47565) in ICC/IF
Immunocytochemistry analysis of MYF5 in immersion fixed C2C12 mouse myoblast cell line undifferentiated (upper panel) or differentiated with horse serum for 7 days (lower panel). Samples were incubated in MYF5 polyclonal antibody (Product # PA5-47565) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Product Details
PA5-47565
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human MYF-5 Met1-Leu255
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609454
Product Specific Information
This antibody detects recombinant human MYF-5 in direct ELISAs and Western blots.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Myogenic factor 5 (MYF5) is a gene located on chromosome 12q21.31, encoding a basic helix-loop-helix (bHLH) transcription factor essential for skeletal muscle development and differentiation. MYF5 is one of the key members of the myogenic regulatory factor (MRF) family, which also includes MyoD, myogenin, and MRF4. It plays a crucial role in myogenesis by regulating the expression of genes necessary for muscle precursor cell proliferation and differentiation into mature muscle fibers. During embryonic development, MYF5 is involved in the formation of muscle tissue and maintenance of muscle progenitor cells. Mutations or dysregulation in MYF5 can lead to developmental abnormalities in muscle formation and are implicated in muscle-related disorders. Research into MYF5 continues to explore its regulatory mechanisms in muscle biology and potential therapeutic applications in muscular dystrophies and muscle repair processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Related Products for
Western Blot
Bioinformatics
Protein Aliases: bHLHc2; Class C basic helix-loop-helix protein 2; Myf-5; Myogenic factor 5; myogenic regulatory factor MYF-5
Gene Aliases: B130010J22Rik; BHLHC2; Myf-5; MYF5
UniProt ID: (Human) P13349, (Mouse) P24699
Entrez Gene ID: (Human) 4617, (Mouse) 17877
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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