Invitrogen

MYOD Monoclonal Antibody (5.8A)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

14 Published Figures

31 References

View all (48) MYOD antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MYOD Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

MYOD Antibody (MA1-41017) in ICC/IF

Immunofluorescence analysis of MYOD was performed using 70% confluent log phase differentiated C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MYOD monoclonal antibody (Product # MA1-41017) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjuga... View More

MYOD Antibody in Immunohistochemistry (Frozen) (IHC (F))

MYOD Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

MYOD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

MYOD Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MYOD Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells lines tested owing to their inherent genetic constitution. Relative expression of MYOD was observed in C2C12 cells differentiated to myotubes (3 and 7 days) in comparison to undifferentiated C2C12 using Anti-MYOD Monoclonal Antibody (Product # MA1-41017) in Western Blot. {RE}

Product Details

MA1-41017

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

1 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5.8A

Immunogen

Recombinant mouse MyoD.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2282434

Product Specific Information

The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.

Suggested positive control: Rh-30.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury.

Pharmaceuticals (Basel, Switzerland)

Siu WS,Ma H,Cheng W,Shum WT,Leung PC

MA1-41017 was used in Immunohistochemistry to demonstrate that topical Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR) treatment was effective at facilitating muscle injury repair.

Sat Aug 12 00:00:00 EDT 2023

Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury.

Pharmaceuticals (Basel, Switzerland)

Siu WS,Ma H,Cheng W,Shum WT,Leung PC

MA1-41017 was used in Immunohistochemistry to demonstrate that topical Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR) treatment was effective at facilitating muscle injury repair.

Sat Aug 12 00:00:00 EDT 2023

RNA-Seq analysis of a Pax3-expressing myoblast clone in-vitro and effect of culture surface stiffness on differentiation.

Scientific reports

Richardson L,Wang D,Hughes R,Johnson CA,Peckham M

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunohistochemistry

Fri Feb 18 00:00:00 EST 2022

Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence.

NPJ Regenerative medicine

Rolland TJ,Peterson TE,Singh RD,Rizzo SA,Boroumand S,Shi A,Witt TA,Nagel M,Kisby CK,Park S,Rowe LA,Paradise CR,Becher LRE,Paradise BD,Stalboerger PG,Trabuco EC,Behfar A

MA1-41017 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to describe PEP-based biopotentiation of hydrogels implemented to restore skeletal muscle function and may serve as a promising approach for the nonsurgical management of SUI.

Thu Sep 29 00:00:00 EDT 2022

Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence.

NPJ Regenerative medicine

Rolland TJ,Peterson TE,Singh RD,Rizzo SA,Boroumand S,Shi A,Witt TA,Nagel M,Kisby CK,Park S,Rowe LA,Paradise CR,Becher LRE,Paradise BD,Stalboerger PG,Trabuco EC,Behfar A

Thu Sep 29 00:00:00 EDT 2022

Loss of the Nuclear Envelope Protein LAP1B Disrupts the Myogenic Differentiation of Patient-Derived Fibroblasts.

International journal of molecular sciences

Kayman Kürekçi G,Acar AC,Dinçer PR

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Western Blot

Sun Nov 06 00:00:00 EDT 2022

miRNA mediated downregulation of cyclase-associated protein 1 (CAP1) is required for myoblast fusion.

Frontiers in cell and developmental biology

Singh AK,Rai A,Weber A,Posern G

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Western Blot

Wed Oct 19 00:00:00 EDT 2022

Growth Factors Do Not Improve Muscle Function in Young or Adult mdx Mice.

Biomedicines

Nielsen TL,Hornsyld TM,Pinós T,Brolin C,Vissing J,Krag TO

MA1-41017 was used in Western Blot to study the effects of myogenic growth factors on muscle function in mouse models with muscular degeneration.

Fri Jan 28 00:00:00 EST 2022

Growth Factors Do Not Improve Muscle Function in Young or Adult mdx Mice.

Biomedicines

Nielsen TL,Hornsyld TM,Pinós T,Brolin C,Vissing J,Krag TO

MA1-41017 was used in Western Blot to study the effects of myogenic growth factors on muscle function in mouse models with muscular degeneration.

Fri Jan 28 00:00:00 EST 2022

Muscle injury causes long-term changes in stem-cell DNA methylation.

Proceedings of the National Academy of Sciences of the United States of America

Falick Michaeli T,Sabag O,Fok R,Azria B,Monin J,Nevo Y,Gielchinsky Y,Berman BP,Cedar H,Bergman Y

MA1-41017 was used in Flow cytometry/Cell sorting to demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation.

Tue Dec 27 00:00:00 EST 2022

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

MA1-41017 was used in Immunohistochemistry to show for the first time that Hrs/Hgs is a master regulator that modulates myogenesis at different levels through the control of trafficking, signaling, and degradation pathways.

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

Advanced Glycation End Products Are Retained in Decellularized Muscle Matrix Derived from Aged Skeletal Muscle.

International journal of molecular sciences

Olson LC,Nguyen TM,Heise RL,Boyan BD,Schwartz Z,McClure MJ

MA1-41017 was used in Western Blot to show that deleterious age-dependent modifications to collagen are present in DMM from old muscle.

Tue Aug 17 00:00:00 EDT 2021

Advanced Glycation End Products Are Retained in Decellularized Muscle Matrix Derived from Aged Skeletal Muscle.

International journal of molecular sciences

Olson LC,Nguyen TM,Heise RL,Boyan BD,Schwartz Z,McClure MJ

MA1-41017 was used in Western Blot to show that deleterious age-dependent modifications to collagen are present in DMM from old muscle.

Tue Aug 17 00:00:00 EDT 2021

Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming.

Cell reports

Fallatah B,Shuaib M,Adroub S,Paytuví-Gallart A,Della Valle F,Nadeef S,Lanzuolo C,Orlando V

MA1-41017 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation.

Tue Nov 30 00:00:00 EST 2021

Filopodia powered by class x myosin promote fusion of mammalian myoblasts.

eLife

Hammers DW,Hart CC,Matheny MK,Heimsath EG,Lee YI,Hammer JA,Cheney RE,Sweeney HL

MA1-41017 was used in Immunocytochemistry to demonstrate that these projections are filopodia generated by class X myosin (Myo10), an unconventional myosin motor protein specialized for filopodia.

Tue Sep 14 00:00:00 EDT 2021

Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.

PloS one

Teo WH,Lo JF,Fan YN,Huang CY,Huang TF

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Western Blot

Mon Mar 22 00:00:00 EDT 2021

Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.

PloS one

Teo WH,Lo JF,Fan YN,Huang CY,Huang TF

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Western Blot

Mon Mar 22 00:00:00 EDT 2021

Myogenic marker expression as a function of age and exercise-based therapy in the tongue.

Experimental gerontology

Kletzien H,Kelm-Nelson CA,Wang S,Suzuki M,Connor NP

MA1-41017 was used in Western Blotting to suggest that strengthening of tongue muscles via clinical rehabilitation strategies also increased the expression of SC regenerative markers in the tongue throughout the exercise duration.

Tue Dec 01 00:00:00 EST 2020

MicroRNA-672-5p Identified during Weaning Reverses Osteopenia and Sarcopenia in Ovariectomized Mice.

Molecular therapy. Nucleic acids

Ahmad N,Kushwaha P,Karvande A,Tripathi AK,Kothari P,Adhikary S,Khedgikar V,Mishra VK,Trivedi R

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Western Blot

Fri Mar 01 00:00:00 EST 2019

Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3β Signaling Pathway.

EBioMedicine

Shen C,Zhou J,Wang X,Yu XY,Liang C,Liu B,Pan X,Zhao Q,Song JL,Wang J,Bao M,Wu C,Li Y,Song YH

MA141017 was used in immunohistochemistry - frozen section to implicate 25-hydroxycholesterol as an inducer of muscle wasting

Wed Feb 01 00:00:00 EST 2017

Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3β Signaling Pathway.

EBioMedicine

Shen C,Zhou J,Wang X,Yu XY,Liang C,Liu B,Pan X,Zhao Q,Song JL,Wang J,Bao M,Wu C,Li Y,Song YH

MA141017 was used in immunohistochemistry - frozen section to implicate 25-hydroxycholesterol as an inducer of muscle wasting

Wed Feb 01 00:00:00 EST 2017

Synthetic niche to modulate regenerative potential of MSCs and enhance skeletal muscle regeneration.

Biomaterials

Pumberger M,Qazi TH,Ehrentraut MC,Textor M,Kueper J,Stoltenburg-Didinger G,Winkler T,von Roth P,Reinke S,Borselli C,Perka C,Mooney DJ,Duda GN,Geißler S

MA1-41017 was used in western blot to research modulation of regenerative potential of MSCs and enhancement of skeletal muscle regeneration by a synthetic niche

Mon Aug 01 00:00:00 EDT 2016

Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle.

Endocrinology

Lesmana R,Sinha RA,Singh BK,Zhou J,Ohba K,Wu Y,Yau WW,Bay BH,Yen PM

MA1-41017 was used in western blot to investigate thyroid hormone-mediated autophagy in skeletal muscle

Fri Jan 01 00:00:00 EST 2016

The role of TORC1 in muscle development in Drosophila.

Scientific reports

Hatfield I,Harvey I,Yates ER,Redd JR,Reiter LT,Bridges D

MA1-41017 was used in western blot to determine the role of mTORC1 in myogenesis using drosophila

Mon Apr 13 00:00:00 EDT 2015

CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury.

Stem cells (Dayton, Ohio)

Rahman MM,Ghosh M,Subramani J,Fong GH,Carlson ME,Shapiro LH

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunofluorescence

Sun Jun 01 00:00:00 EDT 2014

CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury.

Stem cells (Dayton, Ohio)

Rahman MM,Ghosh M,Subramani J,Fong GH,Carlson ME,Shapiro LH

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunofluorescence

Sun Jun 01 00:00:00 EDT 2014

Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.

Cancer genetics

Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK

MA1-41017 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion

Mon Aug 01 00:00:00 EDT 2011

MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3.

The Journal of cell biology

Hirai H,Verma M,Watanabe S,Tastad C,Asakura Y,Asakura A

MA1-41017 was used in immunocytochemistry and western blot to study the mechanism by which the MyoD regulates myoblast apoptosis

Mon Oct 18 00:00:00 EDT 2010

CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.

Experimental cell research

Fukada S,Yamamoto Y,Segawa M,Sakamoto K,Nakajima M,Sato M,Morikawa D,Uezumi A,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Yamamoto H

MA1-41017 was used in immunocytochemistry to study the role of CD90-positive cells in producing laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice

Tue Jan 01 00:00:00 EST 2008

Molecular signature of quiescent satellite cells in adult skeletal muscle.

Stem cells (Dayton, Ohio)

Fukada S,Uezumi A,Ikemoto M,Masuda S,Segawa M,Tanimura N,Yamamoto H,Miyagoe-Suzuki Y,Takeda S

MA1-41017 was used in immunocytochemistry to investigate gene expression in quiescent satellite cells of adult skeletal muscle

Mon Oct 01 00:00:00 EDT 2007

ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin.

The Biochemical journal

Zhao Z,Gruszczynska-Biegala J,Zolkiewska A

MA1-41017 was used in western blot to study the effect of ADP-ribosylation on the interaction between integrin alpha7beta1 and laminin

Sat Jan 01 00:00:00 EST 2005

Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.

Experimental cell research

Holst D,Luquet S,Kristiansen K,Grimaldi PA

MA1-41017 was used in western blot to study the roles of PPARdelta and gamma in myoblast transdifferentiation

Fri Aug 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3

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Gene Aliases: AI503393; BHLHC1; MYF3; MYOD; Myod-1; MYOD1; PUM

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UniProt ID: (Human) P15172, (Mouse) P10085

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Entrez Gene ID: (Human) 4654, (Mouse) 17927

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding nuclear hormone receptor binding protein heterodimerization activity E-box binding DNA binding transcription factor activity, sequence-specific DNA binding enzyme binding sequence-specific DNA binding protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

regulation of alternative mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation muscle cell fate commitment positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion negative regulation of myoblast proliferation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development myotube differentiation cell differentiation skeletal muscle tissue regeneration myoblast differentiation positive regulation of transcription, DNA-templated striated muscle cell differentiation cardiac muscle cell differentiation

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MYOD Monoclonal Antibody (5.8A)

MYOD Antibody (MA1-41017) in ICC/IF

Product Details

MA1-41017

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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