Invitrogen

MYOD Monoclonal Antibody (5.8A)

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Antibody Testing Data (3)
Published Figures (15)

MYOD Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

MYOD Antibody (MA5-12902) in ICC/IF

Immunofluorescence analysis of MyoD1 was done on 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MyoD1 (5.8A) Mouse Monoclonal Antibody (Product # MA5-12902) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More

MYOD Antibody in Immunoprecipitation (IP)

MYOD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

MYOD Antibody in Immunohistochemistry (Frozen) (IHC (F))

MYOD Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-12902

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 11 publications 11 publications

Immunoprecipitation (IP)

2 µg/mL

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Bovine, Chicken, Horse, Human, Mouse, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5.8A

Immunogen

Recombinant mouse MyoD1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982953

Product Specific Information

MA5-12902 targets MyoD1 in IF, IM, and IP applications and shows reactivity with Chicken, Human, mouse, and Rat samples.

The MA5-12902 immunogen is recombinant mouse MyoD1 protein.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Primary mouse myoblast metabotropic purinoceptor profiles and calcium signalling differ with their muscle origin and are altered in mdx dystrophinopathy.

Scientific reports

Róg J,Oksiejuk A,Górecki DC,Zabłocki K

MA5-12902 was used in Immunocytochemistry-immunofluorescence to identify significant differences in the pattern of expression and activity of P2Y receptors and the levels of the calcium signalling toolkit" proteins between mdx and wild-type myoblasts isolated from different muscles."

Thu Jun 08 00:00:00 EDT 2023

Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells.

Science advances

Kim I,Ghosh A,Bundschuh N,Hinte L,Petrosyan E,von Meyenn F,Bar-Nur O

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Fri Apr 08 00:00:00 EDT 2022

MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies.

Genes

Budsuren U,Ulaangerel T,Shen Y,Liu G,Davshilt T,Yi M,Bold D,Zhang X,Bai D,Dorjgotov D,Davaakhuu G,Jambal T,Li B,Du M,Dugarjav M,Bou G

MA5-12902 was used in Immunohistochemistry to study the regulatory mechanism of the transforming growth factor-β member, myostatin (MSTN), in equine muscle cells.

Tue Oct 11 00:00:00 EDT 2022

MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies.

Genes

Budsuren U,Ulaangerel T,Shen Y,Liu G,Davshilt T,Yi M,Bold D,Zhang X,Bai D,Dorjgotov D,Davaakhuu G,Jambal T,Li B,Du M,Dugarjav M,Bou G

MA5-12902 was used in Immunohistochemistry to study the regulatory mechanism of the transforming growth factor-β member, myostatin (MSTN), in equine muscle cells.

Tue Oct 11 00:00:00 EDT 2022

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat.

Communications biology

Stout AJ,Mirliani AB,Rittenberg ML,Shub M,White EC,Yuen JSK,Kaplan DL

MA5-12902 was used in Immunocytochemistry-immunoflourescence to offer a simple, affordable, and effective Beefy-9 and Beefy-9+ serum-free media for improving cultured meat research.

Thu Jun 02 00:00:00 EDT 2022

Upregulation of miR181a/miR212 Improves Myogenic Commitment in Murine Fusion-Negative Rhabdomyosarcoma.

Frontiers in physiology

Pozzo E,Giarratana N,Sassi G,Elmastas M,Killian T,Wang CC,Marini V,Ronzoni F,Yustein J,Uyttebroeck A,Sampaolesi M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Tue Aug 24 00:00:00 EDT 2021

Upregulation of miR181a/miR212 Improves Myogenic Commitment in Murine Fusion-Negative Rhabdomyosarcoma.

Frontiers in physiology

Pozzo E,Giarratana N,Sassi G,Elmastas M,Killian T,Wang CC,Marini V,Ronzoni F,Yustein J,Uyttebroeck A,Sampaolesi M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Tue Aug 24 00:00:00 EDT 2021

FOXP1 Interacts with MyoD to Repress its Transcription and Myoblast Conversion.

Journal of cellular signaling

Wright WE,Li C,Zheng CX,Tucker HO

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Western Blot

Thu Feb 11 00:00:00 EST 2021

Myo/Nog cells expressing muscle proteins are present in preretinal membranes from patients with proliferative vitreoretinopathy.

Experimental eye research

Gerhart J,Morrison N,Gugerty L,Telander D,Bravo-Nuevo A,George-Weinstein M

MA5-12902 was used in Immunohistochemistry to investigate the function of Myo/Nog cells in three ocular trauma patients with proliferative vitreoretinopathy.

Sat Aug 01 00:00:00 EDT 2020

Myo/Nog cells are nonprofessional phagocytes.

PloS one

Gerhart J,Gugerty L,Lecker P,Abdalla F,Martin M,Gerhart O,Gerhart C,Johal K,Bernstein J,Spikes J,Mathers K,Bravo-Nuevo A,George-Weinstein M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Wed Sep 30 00:00:00 EDT 2020

Myo/Nog cells are nonprofessional phagocytes.

PloS one

Gerhart J,Gugerty L,Lecker P,Abdalla F,Martin M,Gerhart O,Gerhart C,Johal K,Bernstein J,Spikes J,Mathers K,Bravo-Nuevo A,George-Weinstein M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Wed Sep 30 00:00:00 EDT 2020

Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage.

PloS one

Gerhart J,Bowers J,Gugerty L,Gerhart C,Martin M,Abdalla F,Bravo-Nuevo A,Sullivan JT,Rimkunas R,Albertus A,Casta L,Getts L,Getts R,George-Weinstein M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunohistochemistry

Tue Sep 08 00:00:00 EDT 2020

Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage.

PloS one

Gerhart J,Bowers J,Gugerty L,Gerhart C,Martin M,Abdalla F,Bravo-Nuevo A,Sullivan JT,Rimkunas R,Albertus A,Casta L,Getts L,Getts R,George-Weinstein M

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunohistochemistry

Tue Sep 08 00:00:00 EDT 2020

Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins.

PloS one

Gerhart J,Behling K,Paessler M,Milton L,Bramblett G,Garcia D,Pitts M,Hurtt R,Crawford M,Lackman R,Nguyen D,Infanti J,FitzGerald P,George-Weinstein M

MA5-12902 was used in Immunohistochemistry-immunofluorescence to screen rhabdomyosarcoma and Wilms tumours for a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins.

Thu Dec 19 00:00:00 EST 2019

Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins.

PloS one

Gerhart J,Behling K,Paessler M,Milton L,Bramblett G,Garcia D,Pitts M,Hurtt R,Crawford M,Lackman R,Nguyen D,Infanti J,FitzGerald P,George-Weinstein M

Thu Dec 19 00:00:00 EST 2019

WNT/β-catenin signaling plays a crucial role in myoblast fusion through regulation of nephrin expression during development.

Development (Cambridge, England)

Suzuki A,Minamide R,Iwata J

MA5-12902 was used in Immunohistochemistry to show that WNT/β-catenin signaling is crucial for myoblast fusion through regulation of the nephrin gene in the Myog-Cre-expressing myoblast population.

Tue Nov 27 00:00:00 EST 2018

Isolation, cultivation and immunostaining of single myofibers: An improved approach to study the behavior of satellite cells.

Journal of biological methods

Lim CL,Ling KH,Cheah PS

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Immunocytochemistry

Sat Nov 04 00:00:00 EDT 2023

PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis.

eLife

Kendall GC,Watson S,Xu L,LaVigne CA,Murchison W,Rakheja D,Skapek SX,Tirode F,Delattre O,Amatruda JF

Published figure using MYOD monoclonal antibody (Product # MA5-12902) in Western Blot

Tue Jun 05 00:00:00 EDT 2018

Inflammation-associated miR-155 activates differentiation of muscular satellite cells.

PloS one

Onodera Y,Teramura T,Takehara T,Itokazu M,Mori T,Fukuda K

MA5-12902 was used in Western Blotting to study the expression of microRNA-155, in muscular satellite cells of aged muscles.

Fri Mar 22 00:00:00 EDT 2019

The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 Ubiquitin Ligase Activity.

Molecular cell

Feng Q,Jagannathan S,Bradley RK

MA5-12902 was used in Western Blotting to study the role of UPF1 in regulated protein decay.

Thu Jul 20 00:00:00 EDT 2017

Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.

The Journal of physiology

Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR

MA5-12902 was used in immunohistochemistry - frozen section to elucidate the role of SMAD7 in muscle

Mon Jun 01 00:00:00 EDT 2015

Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.

The Journal of physiology

Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR

MA5-12902 was used in immunohistochemistry - frozen section to elucidate the role of SMAD7 in muscle

Mon Jun 01 00:00:00 EDT 2015

Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.

Cancer genetics

Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK

MA5-12902 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion

Mon Aug 01 00:00:00 EDT 2011

CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.

Experimental cell research

Fukada S,Yamamoto Y,Segawa M,Sakamoto K,Nakajima M,Sato M,Morikawa D,Uezumi A,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Yamamoto H

MA5-12902 was used in immunocytochemistry to study the role of CD90-positive cells in producing laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice

Tue Jan 01 00:00:00 EST 2008

Molecular signature of quiescent satellite cells in adult skeletal muscle.

Stem cells (Dayton, Ohio)

Fukada S,Uezumi A,Ikemoto M,Masuda S,Segawa M,Tanimura N,Yamamoto H,Miyagoe-Suzuki Y,Takeda S

MA5-12902 was used in immunocytochemistry to investigate gene expression in quiescent satellite cells of adult skeletal muscle

Mon Oct 01 00:00:00 EDT 2007

ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin.

The Biochemical journal

Zhao Z,Gruszczynska-Biegala J,Zolkiewska A

MA5-12902 was used in western blot to study the effect of ADP-ribosylation on the interaction between integrin alpha7beta1 and laminin

Sat Jan 01 00:00:00 EST 2005

Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.

Experimental cell research

Holst D,Luquet S,Kristiansen K,Grimaldi PA

MA5-12902 was used in western blot to study the roles of PPARdelta and gamma in myoblast transdifferentiation

Fri Aug 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myoblast determination protein 1 homolog; MYOD1 homolog; myogenic factor 1; Myogenic factor 3; unnamed protein product

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Gene Aliases: AI503393; BHLHC1; CMD1; MYF3; MYOD; Myod-1; MYOD1; PUM

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UniProt ID: (Human) P15172, (Chicken) P16075, (Mouse) P10085, (Rat) Q02346

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Entrez Gene ID: (Human) 4654, (Chicken) 374048, (Mouse) 17927, (Rat) 337868

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding nuclear hormone receptor binding protein heterodimerization activity E-box binding RNA polymerase II transcription factor activity, sequence-specific DNA binding identical protein binding bHLH transcription factor binding protein dimerization activity promoter-specific chromatin binding DNA binding transcription factor activity, sequence-specific DNA binding enzyme binding sequence-specific DNA binding basic helix-loop-helix transcription factor

Process(es)

regulation of alternative mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation muscle cell fate commitment positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion negative regulation of myoblast proliferation positive regulation of snRNA transcription by RNA polymerase II transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development myotube differentiation cell differentiation skeletal muscle tissue regeneration myoblast differentiation positive regulation of transcription, DNA-templated striated muscle cell differentiation cardiac muscle cell differentiation skeletal muscle atrophy response to muscle stretch

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MYOD Monoclonal Antibody (5.8A)

MYOD Antibody (MA5-12902) in ICC/IF

Product Details

MA5-12902

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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