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Product Details
MA5-32641
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM10-72
Immunogen
Synthetic peptide within Human MyoD1 aa 36-80
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809918
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3
Gene Aliases: BHLHC1; MYF3; MYOD; MYOD1; PUM
UniProt ID: (Human) P15172
Entrez Gene ID: (Human) 4654
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
transcription factor binding
chromatin DNA binding
ubiquitin protein ligase binding
nuclear hormone receptor binding
protein heterodimerization activity
E-box binding
basic helix-loop-helix transcription factor
regulation of alternative mRNA splicing, via spliceosome
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
muscle organ development
myoblast fate determination
skeletal muscle tissue development
myoblast fusion
cellular response to starvation
myotube cell development
myotube differentiation involved in skeletal muscle regeneration
skeletal muscle cell differentiation
muscle cell fate commitment
positive regulation of skeletal muscle tissue regeneration
regulation of RNA splicing
skeletal muscle fiber adaptation
histone H3 acetylation
histone H4 acetylation
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
cellular response to glucocorticoid stimulus
cellular response to estradiol stimulus
cellular response to oxygen levels
positive regulation of myoblast fusion
negative regulation of myoblast proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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