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Application
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Canine IgE Secondary Antibody (SA5-10300) in ELISA
ELISA analysis was performed using Dog IgE antigen (500 ng/well, 60 min). Samples were blocked for 60 min with 1% BSA, incubated with Mouse anti-Dog IgE (60 min), and followed by HRP Goat anti-Mouse IgG h+l (Product # SA5-10300) for 30 min. Development was done with TMB substrate (10 min) and the reaction was stopped with 0.3M H2SO4. Results were read on a microplate reader at OD450.
Product Details
SA5-10300
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
ELISA (ELISA)
Assay-dependent
Product Specifications
Species Reactivity
Dog
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
E671
Immunogen
Canine IgE
Conjugate
Unconjugated
Form
Liquid
Concentration
3.96 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2868348
Target
IgE
Antibody Form
Whole Antibody
Target Information
Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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