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Invitrogen
N6-Methyladenosine (m6A) Recombinant Rabbit Monoclonal Antibody (RM362)
Application
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N6-Methyladenosine (m6A) Antibody (MA5-33030) in DB
Dot blot analysis of Lambda DNA without or with m6A, using N6-Methyladenosine (m6A) Recombinant Monoclonal antibody (Product # MA5-33030). The membrane was pre-spotted with 5, and 0.5 ng/dot of lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-).
Product Details
MA5-33030
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM362
Immunogen
BSA-conjugated N6-Methyladenosine.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810123
Product Specific Information
Specificity: reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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