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NAG-1 Antibody (600-401-B10) in WB
Western blot using Rocklands affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.
Product Details
600-401-B10
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody specifically reacts with the amino terminal end of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Multimeric forms of NAG-1 may be detected. Cross-reactivity with NAG-1 from other sources has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, whose members play an important role during prenatal development and postnatal growth, and the remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in the placenta and, to a much lesser extent, in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest GDF-15's involvement in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GDF-15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NAG-1; NAG1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta
Gene Aliases: GDF-15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB
UniProt ID: (Human) Q99988
Entrez Gene ID: (Human) 9518
signal transduction
transforming growth factor beta receptor signaling pathway
cell-cell signaling
positive regulation of pathway-restricted SMAD protein phosphorylation
regulation of apoptotic process
regulation of MAPK cascade
cell development
SMAD protein signal transduction
positive regulation of myoblast fusion
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