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            • Primary Antibodies ›
            • NCKAP1L Antibodies

            Invitrogen

            NCKAP1L Polyclonal Antibody

            2 Published Figures
            2 References
            View all (4) NCKAP1L antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # PA5-58813
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            Price (USD)
            494.00
            100 µL
             
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            Datasheet
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            Cite NCKAP1L Polyclonal Antibody

            • Antibody Testing Data (4)
            • Published Figures (2)
            Application
            NCKAP1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 6

            NCKAP1L Antibody (PA5-58813) in IHC (P)

            Immunohistochemical analysis of NCKAP1L in human lymph node using NCKAP1L Polyclonal Antibody (Product # PA5-58813) shows strong cytoplasmic positivity in non-germinal center cells.
            View Product

            NCKAP1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            NCKAP1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            NCKAP1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            NCKAP1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            NCKAP1L Antibody in Western Blot (WB)
            NCKAP1L Antibody in Western Blot (WB)

            Product Details

            PA5-58813

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.04-0.4 µg/mL
            View 2 publications 2 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:200-1:500
            -
            Product Specifications

            Species Reactivity

            Human

            Published species

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Recombinant protein corresponding to Human NCKAP1L. Recombinant protein control fragment (Product #RP-97719).
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.2, with 40% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2644516

            Product Specific Information

            Immunogen sequence: VLIRMYNIKK TCSDPKSKPP FLLEKSMEPS LKYINKKFPN IDVRNSTQHL GPVHREKAEI IRFLTNYYQS FVD

            Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.

            Target Information

            This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010].

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Hematopoietic protein 1; Membrane-associated protein HEM-1; Nck-associated protein 1-like

            View more View less

            Gene Aliases: HEM1; NCKAP1L

            View more View less

            UniProt ID: (Human) P55160

            View more View less

            Entrez Gene ID: (Human) 3071

            View more View less

            Function(s)

            GTPase activator activity protein binding protein kinase activator activity protein complex binding

            Process(es)

            B cell homeostasis myeloid cell homeostasis protein complex assembly chemotaxis maintenance of cell polarity neutrophil chemotaxis positive regulation of actin filament polymerization cortical actin cytoskeleton organization positive regulation of B cell proliferation activation of protein kinase activity negative regulation of interleukin-17 production negative regulation of interleukin-6 production positive regulation of cell adhesion mediated by integrin negative regulation of myosin-light-chain-phosphatase activity Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of T cell proliferation positive regulation of phosphorylation response to drug T cell homeostasis negative regulation of apoptotic process positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of CD8-positive, alpha-beta T cell differentiation positive regulation of GTPase activity positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation positive regulation of erythrocyte differentiation vascular endothelial growth factor receptor signaling pathway erythrocyte development B cell receptor signaling pathway positive regulation of phagocytosis, engulfment actin polymerization-dependent cell motility positive regulation of neutrophil chemotaxis
            It has to be done as per old AB suggested Products section.
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