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            • Primary Antibodies ›
            • NEK6 Antibodies

            Proteintech

            NEK6 Polyclonal Antibody

            2 References
            View all (25) NEK6 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # 10378-1-AP
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            Price (USD)
            492.00
            150 µL
             
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            Cite NEK6 Polyclonal Antibody

            • Antibody Testing Data (8)
            Application
            NEK6 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 8

            NEK6 Antibody (10378-1-AP) in ICC/IF

            Immunofluorescent analysis of ( -20°C Ethanol ) fixed COS-7 cells using 10378-1-AP (NEK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
            View Product

            NEK6 Antibody in Immunocytochemistry (ICC/IF)
            NEK6 Antibody in Immunocytochemistry (ICC/IF)
            NEK6 Antibody in Western Blot (WB)
            NEK6 Antibody in Western Blot (WB)
            NEK6 Antibody in Western Blot (WB)
            NEK6 Antibody in Western Blot (WB)
            NEK6 Antibody in Western Blot (WB)
            NEK6 Antibody in Western Blot (WB)

            Product Details

            10378-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:1,000
            View 2 publications 2 publications

            Immunocytochemistry (ICC/IF)

            1:20-1:200
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Non-human primate, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            NEK6 Fusion Protein Ag0266 (6-209 aa encoded by BC000101)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            Immunogen sequence: GHMPHGGSS NNLCHTLGPV HPPDPQRHPN TLSFRCSLAD FQIEKKIGRG QFSEVYKATC LLDRKTVALK KVQIFEMMDA KARQDCVKEI GLLKQLNHPN IIKYLDSFIE DNELNIVLEL ADAGDLSQMI KYFKKQKRLI PERTVWKYFV QLCSAVEHMH SRRVMHRDIK PANVFITATG VVKLGDLGLG RFFSSETTAA HSLVG (6-209 aa encoded by BC000101)

            Target Information

            NEK6 kinase is a member of the NEK family. NEK6 is a serine/threonine kinase that controls the initiation of mitosis and is required for the metaphase-anaphase transition. The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Never in mitosis A-related kinase 6; NIMA (never in mitosis gene a)-related expressed kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related expressed kinase 6; NIMA-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512; putative serine-threonine protein kinase; RP11-101K10.6; Serine/threonine-protein kinase Nek6; SID

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            Gene Aliases: 1300007C09Rik; NEK6; SID6-1512

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            UniProt ID: (Human) Q9HC98, (Mouse) Q9ES70, (Rat) P59895

            View more View less

            Entrez Gene ID: (Human) 10783, (Mouse) 59126, (Rat) 360161

            View more View less

            Function(s)

            magnesium ion binding protein serine/threonine kinase activity signal transducer activity protein binding ATP binding kinesin binding protein kinase binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

            Process(es)

            cytokinesis protein phosphorylation apoptotic process chromosome segregation mitotic nuclear division mitotic nuclear envelope disassembly signal transduction regulation of mitotic cell cycle peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition G2 DNA damage checkpoint positive regulation of I-kappaB kinase/NF-kappaB signaling protein autophosphorylation spindle assembly regulation of cellular senescence cell cycle regulation of signal transduction phosphorylation cell division
            It has to be done as per old AB suggested Products section.
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