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Bethyl Laboratories
NF1 Polyclonal Antibody
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Application
FIGURE: 1 / 3
NF1 Antibody (A300-140A) in WB
Detection of human Neurofibromin (NF1) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, MDA-MB-435, and HT-29 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Neurofibromin (NF1) antibody (Product # A300-140A lot 4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A300-140A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 2760 and the C-terminus (residue 2818) of human Neurofibromin 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686J1293; FLJ21220; Neurofibromatosis-related protein NF-1; Neurofibromin
Gene Aliases: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS
UniProt ID: (Human) P21359, (Mouse) Q04690
Entrez Gene ID: (Human) 4763, (Mouse) 18015
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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