Invitrogen

NFAT5 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

30 Published Figures

30 References

View all (14) NFAT5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NFAT5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 44

NFAT5 Antibody (PA1-023) in ICC/IF

Immunofluorescent analysis of NFAT5 using anti-NFAT5 polyclonal antibody (Product # PA1-023) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFAT5 (Product # PA1-023), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goa... View More

NFAT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFAT5 Antibody in Immunoprecipitation (IP)

NFAT5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

NFAT5 Antibody — Figure 2 NFAT does not appear to be involved in latent HIV transcription in 5A8 cells. A, immunoblotting analysis of RelA and NFAT knockdowns in 5A8 cells. Negative control siRNA (lanes 1 and 6), siRNAs against RelA (lanes 2 and 7), NFATc1, NFATc2, and NFATc3 (lanes 3 and 8), NFAT5 alone (lanes 4 and 9), and all four NFATs (lanes 5 and 10) were introduced into 5A8 cells by Amaxa nucleofection twice within 48 h. Cells were then treated with DMSO (lanes 1 to 5) or 500 nM CsA (lanes 6 to 10) for 2 h, stimulated with anti-CD3 and anti-CD28 antibodies for 24 h, and collected for immunoblotting or FACS analyses. CsA reduced the levels of NFATc1 and NFATc2, which autoamplified their expressions after TCR crosslinking. B, FACS analysis of LTR-driven GFP reporter activity ( y-axis ). Values are mean +- SEM from four experiments. Reporter activity remained partially sensitive to CsA inhibition (lanes 6 to 10) but was insensitive to NFAT knockdown (lanes 3 to 5). C, characterization of 5A8-NFAT-DsRed2 cells. Cells were pretreated with DMSO ( panels 1-3 ) or 500 nM CsA for 2 h ( panel 4 ) and incubated with 20 nM PMA ( panel 2 ), or 20 nM PMA and 2 uM ionomycin ( panels 3 and 4 ) for 24 h, followed by FACS analysis. PMA alone induced activity of the GFP reporter but not the DsRed2 reporter ( panel 2 ). Ionomycin and PMA induced both reporters ( panel 3 ). CsA fully inhibited combined PMA/ionomycin induction of DsRed2 reporter but only partially suppressed GFP reporter activity to levels {KD}

Product Details

PA1-023

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

3 µg

ChIP assay (ChIP)

View 2 publications 2 publications

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2152617

Product Specific Information

PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.

PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.

The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.

Target Information

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interleukin-20 is involved in dry eye disease and is a potential therapeutic target.

Journal of biomedical science

Wang HH,Chen WY,Huang YH,Hsu SM,Tsao YP,Hsu YH,Chang MS

PA1-023 was used in Western Blotting to demonstrate the functions of IL-20 in DED and presented a potential therapeutic option for this condition.

Thu Jun 09 00:00:00 EDT 2022

Rac1 deficiency impairs postnatal development of the renal papilla.

Scientific reports

Ayuzawa N,Nishimoto M,Kawarazaki W,Oba S,Marumo T,Aiba A,Fujita T

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Thu Nov 24 00:00:00 EST 2022

Aestivation motifs explain hypertension and muscle mass loss in mice with psoriatic skin barrier defect.

Acta physiologica (Oxford, England)

Wild J,Jung R,Knopp T,Efentakis P,Benaki D,Grill A,Wegner J,Molitor M,Garlapati V,Rakova N,Markó L,Marton A,Mikros E,Münzel T,Kossmann S,Rauh M,Nakano D,Kitada K,Luft F,Waisman A,Wenzel P,Titze J,Karbach S

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus.

Frontiers in endocrinology

Kim DH,Kim KK,Lee TH,Eom H,Kim JW,Park JW,Jeong JK,Lee BJ

PA1-023 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, ChIP Assay to report a role for tonicity-responsive enhancer binding protein (TonEBP), a transcription factor sensitive to cellular tonicity, in regulating osmosensitive hypothalamic AVP gene transcription.

Thu Dec 16 00:00:00 EST 2021

Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus.

Frontiers in endocrinology

Kim DH,Kim KK,Lee TH,Eom H,Kim JW,Park JW,Jeong JK,Lee BJ

Thu Dec 16 00:00:00 EST 2021

Impact of salt and the osmoprotective transcription factor NFAT-5 on macrophages during mechanical strain.

Immunology and cell biology

Schröder A,Leikam A,Käppler P,Neubert P,Jantsch J,Neuhofer W,Deschner J,Proff P,Kirschneck C

PA1-023 was used in Western Blotting to investigate the role of NFAT-5 in these responses, NFAT-5 was both constitutively expressed and silenced. Salt and compressive strain, but not tensile strain increased the expression of NFAT-5 and most tested inflammatory factors in macrophages.

Fri Jan 01 00:00:00 EST 2021

Impact of salt and the osmoprotective transcription factor NFAT-5 on macrophages during mechanical strain.

Immunology and cell biology

Schröder A,Leikam A,Käppler P,Neubert P,Jantsch J,Neuhofer W,Deschner J,Proff P,Kirschneck C

Fri Jan 01 00:00:00 EST 2021

NFAT5 Amplifies Antipathogen Responses by Enhancing Chromatin Accessibility, H3K27 Demethylation, and Transcription Factor Recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Lunazzi G,Buxadé M,Riera-Borrull M,Higuera L,Bonnin S,Huerga Encabo H,Gaggero S,Reyes-Garau D,Company C,Cozzuto L,Ponomarenko J,Aramburu J,López-Rodríguez C

PA1-023 was used in Western Blot to advance our understanding about specific mechanisms that optimize antipathogen responses to limit infections.

Tue Jun 01 00:00:00 EDT 2021

Na⁺ _i/K⁺ _i imbalance contributes to gene expression in endothelial cells exposed to elevated NaCl.

Heliyon

Fedorov DA,Sidorenko SV,Yusipovich AI,Parshina EY,Tverskoi AM,Abramicheva PA,Maksimov GV,Orlov SN,Lopina OD,Klimanova EA

PA1-023 was used in Western Blotting to study early responses of endothelial cells caused by a moderate increase in extracellular sodium concentration.

Wed Sep 01 00:00:00 EDT 2021

Na⁺ _i/K⁺ _i imbalance contributes to gene expression in endothelial cells exposed to elevated NaCl.

Heliyon

Fedorov DA,Sidorenko SV,Yusipovich AI,Parshina EY,Tverskoi AM,Abramicheva PA,Maksimov GV,Orlov SN,Lopina OD,Klimanova EA

PA1-023 was used in Western Blotting to study early responses of endothelial cells caused by a moderate increase in extracellular sodium concentration.

Wed Sep 01 00:00:00 EDT 2021

Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity.

International journal of molecular sciences

Schröder A,Gubernator J,Leikam A,Nazet U,Cieplik F,Jantsch J,Neubert P,Titze J,Proff P,Kirschneck C

PA1-023 was used in Western Blotting to investigate the impact of salt and NFAT-5 on osteoclast activity and orthodontic tooth movement (OTM).

Sat Jan 09 00:00:00 EST 2021

NCX1 represents an ionic Na+ sensing mechanism in macrophages.

PLoS biology

Neubert P,Homann A,Wendelborn D,Bär AL,Krampert L,Trum M,Schröder A,Ebner S,Weichselbaum A,Schatz V,Linz P,Veelken R,Schulte-Schrepping J,Aschenbrenner AC,Quast T,Kurts C,Geisberger S,Kunzelmann K,Hammer K,Binger KJ,Titze J,Müller DN,Kolanus W,Schultze JL,Wagner S,Jantsch J

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Mon Jun 01 00:00:00 EDT 2020

NCX1 represents an ionic Na+ sensing mechanism in macrophages.

PLoS biology

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Mon Jun 01 00:00:00 EDT 2020

Recurrent Amplification of the Osmotic Stress Transcription Factor NFAT5 in Adrenocortical Carcinoma.

Journal of the Endocrine Society

Brown TC,Nicolson NG,Man J,Gibson CE,Stenman A,Juhlin CC,Korah R,Carling T

PA1-023 was used in Immunohistochemistry to test whether NFAT5 expression correlated with ion/osmolyte channel and regulatory protein expression patterns in adrenocortical carcinoma.

Wed Jul 01 00:00:00 EDT 2020

HIF1A and NFAT5 coordinate Na⁺-boosted antibacterial defense via enhanced autophagy and autolysosomal targeting.

Autophagy

Neubert P,Weichselbaum A,Reitinger C,Schatz V,Schröder A,Ferdinand JR,Simon M,Bär AL,Brochhausen C,Gerlach RG,Tomiuk S,Hammer K,Wagner S,van Zandbergen G,Binger KJ,Müller DN,Kitada K,Clatworthy MR,Kurts C,Titze J,Abdullah Z,Jantsch J

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Fri Nov 01 00:00:00 EDT 2019

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

PA1-023 was used in Western Blot to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 EDT 2017

Hyperosmotic stress enhances cytotoxicity of SMAC mimetics.

Cell death & disease

Bittner S,Knoll G,Ehrenschwender M

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Thu Aug 03 00:00:00 EDT 2017

Tumour exosome integrins determine organotropic metastasis.

Nature

Hoshino A,Costa-Silva B,Shen TL,Rodrigues G,Hashimoto A,Tesic Mark M,Molina H,Kohsaka S,Di Giannatale A,Ceder S,Singh S,Williams C,Soplop N,Uryu K,Pharmer L,King T,Bojmar L,Davies AE,Ararso Y,Zhang T,Zhang H,Hernandez J,Weiss JM,Dumont-Cole VD,Kramer K,Wexler LH,Narendran A,Schwartz GK,Healey JH,Sandstrom P,Labori KJ,Kure EH,Grandgenett PM,Hollingsworth MA,de Sousa M,Kaur S,Jain M,Mallya K,Batra SK,Jarnagin WR,Brady MS,Fodstad O,Muller V,Pantel K,Minn AJ,Bissell MJ,Garcia BA,Kang Y,Rajasekhar VK,Ghajar CM,Matei I,Peinado H,Bromberg J,Lyden D

PA1-023 was used in western blot to analyze organotropic metasistasis and tumour exosome integrins

Thu Nov 19 00:00:00 EST 2015

Arterial wall stress controls NFAT5 activity in vascular smooth muscle cells.

Journal of the American Heart Association

Scherer C,Pfisterer L,Wagner AH,Hödebeck M,Cattaruzza M,Hecker M,Korff T

PA1-023 was used in ChIP assay to study the mechanisms by which arterial wall mechanical stretch leads to activation of vascular smooth muscle cell NFAT5

Mon Mar 10 00:00:00 EDT 2014

NFAT5 represses canonical Wnt signaling via inhibition of β-catenin acetylation and participates in regulating intestinal cell differentiation.

Cell death & disease

Wang Q,Zhou Y,Rychahou P,Liu C,Weiss HL,Evers BM

PA1-023 was used in immunohistochemistry and western blot to study the role of beta-catenin acetylation in the mechanism by which NFAT5 modulates Wnt signaling and the significance for NFAT5 regulation of intestinal cell differentiation

Thu Jun 13 00:00:00 EDT 2013

NFAT5 represses canonical Wnt signaling via inhibition of β-catenin acetylation and participates in regulating intestinal cell differentiation.

Cell death & disease

Wang Q,Zhou Y,Rychahou P,Liu C,Weiss HL,Evers BM

Thu Jun 13 00:00:00 EDT 2013

Calcium/calcineurin synergizes with prostratin to promote NF-κB dependent activation of latent HIV.

PloS one

Chan JK,Bhattacharyya D,Lassen KG,Ruelas D,Greene WC

PA1-023 was used in western blot to study NFkB-dependent activation of latent HIV infection and the synergistic role of calcineurin and prostratin

Tue Aug 05 00:00:00 EDT 2014

NFAT5 regulates the canonical Wnt pathway and is required for cardiomyogenic differentiation.

Biochemical and biophysical research communications

Adachi A,Takahashi T,Ogata T,Imoto-Tsubakimoto H,Nakanishi N,Ueyama T,Matsubara H

PA1-023 was used in western blot to study the cardiomyogenic differentiation of stem cells and the role played by NFAT5

Fri Sep 28 00:00:00 EDT 2012

Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses.

Early human development

Figueroa H,Lozano M,Suazo C,Eixarch E,Illanes SE,Carreño JE,Villanueva S,Hernández-Andrade E,Gratacós E,Irarrazabal CE

PA1-023 was used in western blot to study the effects on fetal rabbit renal gene expression of intrauterine growth restriction

Thu Nov 01 00:00:00 EDT 2012

NFAT5 is activated by hypoxia: role in ischemia and reperfusion in the rat kidney.

PloS one

Villanueva S,Suazo C,Santapau D,Pérez F,Quiroz M,Carreño JE,Illanes S,Lavandero S,Michea L,Irarrazabal CE

PA1-023 was used in immunohistochemistry and western blot to study the induction of NFAT5 expression and activity by hypoxia and the significance for renal ischemic injury

Tue Nov 13 00:00:00 EST 2012

Contribution of SHP-1 protein tyrosine phosphatase to osmotic regulation of the transcription factor TonEBP/OREBP.

Proceedings of the National Academy of Sciences of the United States of America

Zhou X,Gallazzini M,Burg MB,Ferraris JD

PA1-023 was used in western blot to identify the phosphatase regulating responses to hypertonicity

Tue Apr 13 00:00:00 EDT 2010

Mediator of DNA damage checkpoint 1 (MDC1) contributes to high NaCl-induced activation of the osmoprotective transcription factor TonEBP/OREBP.

PloS one

Kunin M,Dmitrieva NI,Gallazzini M,Shen RF,Wang G,Burg MB,Ferraris JD

PA1-023 was used in western blot to investigate the role of MDC1 in the activation of osmoprotective transcription factor TonEBP/OREBP by high salt concentration

Wed Aug 11 00:00:00 EDT 2010

Integrin alpha6beta4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin.

The Journal of biological chemistry

Chen M,Sinha M,Luxon BA,Bresnick AR,O'Connor KL

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Fri Jan 16 00:00:00 EST 2009

Integrin alpha6beta4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin.

The Journal of biological chemistry

Chen M,Sinha M,Luxon BA,Bresnick AR,O'Connor KL

Published figure using NFAT5 polyclonal antibody (Product # PA1-023) in Western Blot

Fri Jan 16 00:00:00 EST 2009

The transcription factor NFAT5 is required for cyclin expression and cell cycle progression in cells exposed to hypertonic stress.

PloS one

Drews-Elger K,Ortells MC,Rao A,López-Rodriguez C,Aramburu J

PA1-023 was used in western blot to investigate the role of NFAT5 in cyclin expression and cell proliferation under hypertonic conditions

Tue Jul 14 00:00:00 EDT 2009

The transcription factor NFAT5 is required for cyclin expression and cell cycle progression in cells exposed to hypertonic stress.

PloS one

Drews-Elger K,Ortells MC,Rao A,López-Rodriguez C,Aramburu J

PA1-023 was used in western blot to investigate the role of NFAT5 in cyclin expression and cell proliferation under hypertonic conditions

Tue Jul 14 00:00:00 EDT 2009

Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice.

BMC molecular biology

Morancho B,Minguillón J,Molkentin JD,López-Rodríguez C,Aramburu J

PA1-023 was used in western blot to study the activation of NFAT5 in primary cells and its response to hypertonicity

Fri Jan 25 00:00:00 EST 2008

Phosphatidylinositol 3-kinase mediates activation of ATM by high NaCl and by ionizing radiation: Role in osmoprotective transcriptional regulation.

Proceedings of the National Academy of Sciences of the United States of America

Irarrazabal CE,Burg MB,Ward SG,Ferraris JD

PA1-023 was used in western blot to investigate the role of phosphatidylinositol 3-kinase in NaCl- or ionizing radiation-induced ATM activation.

Tue Jun 06 00:00:00 EDT 2006

Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl.

Proceedings of the National Academy of Sciences of the United States of America

Gallazzini M,Ferraris JD,Kunin M,Morris RG,Burg MB

PA1-023 was used in western blot to demonstrate the effect of high NaCl on neuropathy target esterase and its subsequent effect on the production and accumulation of glycerophosphocholine in mammalian renal cells.

Tue Oct 10 00:00:00 EDT 2006

Expression of osmotic stress-related genes in tissues of normal and hyposmotic rats.

American journal of physiology. Renal physiology

Zhang Z,Ferraris JD,Brooks HL,Brisc I,Burg MB

PA1-023 was used in western blot to study the expression of TONEBP in control and hyposmotic rats

Wed Oct 01 00:00:00 EDT 2003

Activity of the TonEBP/OREBP transactivation domain varies directly with extracellular NaCl concentration.

Proceedings of the National Academy of Sciences of the United States of America

Ferraris JD,Williams CK,Persaud P,Zhang Z,Chen Y,Burg MB

PA1-023 was used in western blot to investigate the tonicity dependence of the transactivation domain activity of TonEBP/OREBP C terminus.

Tue Jan 22 00:00:00 EST 2002

Bioinformatics

Protein Aliases: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; Rel domain-containing transcription factor NFAT5; T-cell transcription factor NFAT5; TonE-binding protein; tonicity-responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein

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Gene Aliases: AI225870; B130038B15Rik; CAG-8; CAG80; KIAA0827; mKIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP

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UniProt ID: (Human) O94916, (Mouse) Q9WV30

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Entrez Gene ID: (Human) 10725, (Mouse) 54446

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding Rel homology transcription factor

Process(es)

cytokine production transcription from RNA polymerase II promoter response to osmotic stress signal transduction excretion positive regulation of transcription from RNA polymerase II promoter regulation of calcineurin-NFAT signaling cascade transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression

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NFAT5 Polyclonal Antibody

NFAT5 Antibody (PA1-023) in ICC/IF

Product Details

PA1-023

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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