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Invitrogen
NKCC2 Polyclonal Antibody
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Application
FIGURE: 1 / 1
NKCC2 Antibody (PA5-88905) in WB
Western blot analysis of NKCC2 in extracts of various cell lines. Samples were incubated with NKCC2 Polyclonal antibody (Product # PA5-88905) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88905
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_0003292)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805209
Product Specific Information
Immunogen sequence: FGDEAQKRLR ISFRPGNQEC YDNFLQSGET AKTDASFHAY DSHTNTYYLQ TFGHNTMDAV PKIEYYRNTG SISGPKVNRP SLLEIHEQLA KNVAVTPSSA D; Positive Samples: Mouse Kidney, Rat Kidney; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BSC1; bumetanide-sensitive cotransporter type 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; MGC48843; mNKCC2; Na-K-2Cl cotransporter 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; Solute carrier family 12 member 1; Solute carrier family 12 member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter); Solute carrier family 12, member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter)
Gene Aliases: AI788571; D630042G03Rik; mBSC1; Nkcc2; Slc12a1; urehr3
UniProt ID: (Mouse) P55014
Entrez Gene ID: (Mouse) 20495, (Rat) 25065
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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