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NLGN3 Antibody (MA5-45698) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Neuroligin 3 monoclonal antibody (Product # MA5-45698) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) P... View More
Product Details
MA5-45698
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N110/29
Immunogen
Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932152
Product Specific Information
Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
1 µg/mL of MA5-45698 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.
This antibody was formerly sold as clone S110-29.
Target Information
This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASPGX1; AUTSX1; Gliotactin homolog; neuroligin 3 protein; Neuroligin-3
Gene Aliases: A230085M13Rik; HNL3; KIAA1480; NL3; NLGN3
UniProt ID: (Human) Q9NZ94, (Mouse) Q8BYM5, (Rat) Q62889
Entrez Gene ID: (Human) 54413, (Mouse) 245537, (Rat) 171297
regulation of respiratory gaseous exchange by neurological system process
receptor-mediated endocytosis
neuron cell-cell adhesion
synapse assembly
learning
visual learning
adult behavior
social behavior
axon extension
oligodendrocyte differentiation
modulation of synaptic transmission
synapse organization
positive regulation of synapse assembly
positive regulation of synaptic transmission, glutamatergic
rhythmic synaptic transmission
excitatory postsynaptic potential
inhibitory postsynaptic potential
long-term synaptic potentiation
positive regulation of dendritic spine development
negative regulation of dendritic spine morphogenesis
vocalization behavior
negative regulation of excitatory postsynaptic potential
postsynaptic membrane assembly
presynaptic membrane assembly
positive regulation of inhibitory postsynaptic potential
regulation of long-term synaptic potentiation
positive regulation of protein localization to synapse
regulation of N-methyl-D-aspartate selective glutamate receptor activity
regulation of terminal button organization
positive regulation of excitatory postsynaptic potential
positive regulation of synaptic vesicle clustering
positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
cell adhesion
regulation of synaptic transmission, glutamatergic
regulation of dendritic spine morphogenesis
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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