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Invitrogen
NLGN4X (Neuroligin-4) Monoclonal Antibody (CSTEM30), Alexa Fluor™ 488, eBioscience™
Application
FIGURE: 1 / 2
NLGN4X (Neuroligin-4) Antibody (53-9158-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL Anti-Human NLGN4X (Neuroligin-4) Alexa Fluor® 488 (right). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.
Product Details
53-9158-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CSTEM30
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2716973
Product Specific Information
This monoclonal antibody CSTEM30 reacts with human Neuroligin-4, X linked, also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X.
Applications Reported: This CSTEM30 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis.
Applications Tested: This CSTEM30 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human IPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.125 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
Neuroligin-4, X linked (also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X) is a postsynaptic cell-adhesion molecule that interacts with presynaptic cell-surface neurexins. These interactions bridge together the synaptic cleft and have an essential role in synaptic transmissions between neurons. There are 5 neuroligin genes in humans: NLGN1, NLGN2, NLGN3, NLGN4X, and NLGN4Y. Mutations in NLGN3 and NLGN4X are linked to Autism Spectrum Disorders (ASD). The expression of neuroligin-4, X-linked has been reported on human pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HNLX; MGC22376; Neuroligin X; Neuroligin-4, X-linked; NLGN
Gene Aliases: ASPGX2; AUTSX2; HLNX; HNL4X; KIAA1260; NLGN4; NLGN4X; UNQ365/PRO701
UniProt ID: (Human) Q8N0W4
Entrez Gene ID: (Human) 57502
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