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            • Primary Antibodies ›
            • NO66 Antibodies

            Bethyl Laboratories

            NO66 Polyclonal Antibody

            View all (8) NO66 antibodies
            Datasheet
            Protocols
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            Catalog # A303-199A-T
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            Cite NO66 Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            NO66 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            NO66 Antibody (A303-199A-T) in WB

            Detection of human NO66 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NO66 antibody (Product # A303-199A) used for WB at 0.1 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. NO66 was also immunoprecipitated by rabbit anti-NO66 antibody A303-198A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and ... View More
            View Product

            NO66 Antibody in Western Blot (WB)

            Product Details

            A303-199A-T

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-10 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 50 and 100 of human Nucleolar Protein 66.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            tris citrate/phosphate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Target Information

            Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. [UniProt]

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: 60S ribosomal protein L8 histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; FLJ21802; histone lysine demethylase NO66; hsNO66; lysine-specific demethylase NO66; MYC-associated protein with JmjC domain; Nucleolar protein 66; Ribosomal oxygenase 1; Ribosomal oxygenase NO66; ROX; up-regulated in lung cancer 2

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            Gene Aliases: C14orf169; hsNO66; MAPJD; NO66; RIOX1; ROX; URLC2

            View more View less

            UniProt ID: (Human) Q9H6W3

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            Entrez Gene ID: (Human) 79697

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            Function(s)

            iron ion binding protein binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors histone demethylase activity (H3-K4 specific) histone demethylase activity (H3-K36 specific)

            Process(es)

            chromatin remodeling transcription, DNA-templated peptidyl-amino acid modification histone H3-K4 demethylation ribosome biogenesis post-translational protein modification negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated oxidation-reduction process histone H3-K36 demethylation
            It has to be done as per old AB suggested Products section.
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