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Application
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NOB1 Antibody (PA5-104321) in IHC (P)
Immunohistochemistry analysis of NOB1 in mouse muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NOB1 polyclonal antibody (Product # PA5-104321) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-104321
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NOB1(Accession Q9ULX3), corresponding to amino acid residues N376-R412.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853631
Product Specific Information
Antibody detects endogenous levels of total NOB1.
Target Information
In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenocarcinoma antigen recognized by T lymphocytes 4; nin one binding protein; NIN1/RPN12 binding protein 1 homolog; Phosphorylation regulatory protein HP-10; Protein ART-4; PSMD8 binding protein 1; RNA-binding protein NOB1
Gene Aliases: 1700021I09Rik; ART-4; ART4; MST158; MSTP158; NOB1; NOB1P; PSMD8BP1
UniProt ID: (Human) Q9ULX3, (Rat) Q6VEU1, (Mouse) Q8BW10
Entrez Gene ID: (Human) 28987, (Rat) 291996, (Mouse) 67619
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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