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Invitrogen

NOD2 Polyclonal Antibody

Advanced Verification

1 Reference

View all (14) NOD2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (1)

Application

NOD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

NOD2 Antibody (PA5-83584) in IHC (P)

Immunohistochemical analysis of NOD2 in human skin using NOD2 Polyclonal Antibody (Product # PA5-83584) shows moderate to strong cytoplasmic positivity in keratinocytes.

Product Image Gallery

NOD2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of NOD2 demonstrates antibody specificity. Immunohistochemical analysis of NOD2 using anti-NOD2 Polyclonal Antibody (Product #PA5-83584), shows significant staining of NOD2 in skin and shows minimal or weak staining in kidney tissues. The relative expression levels of NOD2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-83584

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NOD2. Recombinant protein control fragment (Product #RP-98741).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2790737

Product Specific Information

Immunogen sequence: VWNKGTWACQ KLIAAAQEAQ ADSQSPKLHG CWDPHSLHPA RDLQSHRPAI VRRLHSHVEN MLDLAWERGF VSQYECDEIR LPI

Target Information

NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Solute carrier family 12 member 8 impacts the biological behaviors of breast carcinoma cells by activating TLR/NLR signaling pathway.

Cytotechnology

Li L,Xia J,Cui R,Kong B

PA5-83584 was used in Immunohistochemistry (Paraffin), Western Blot to suggest that SLC12A8 plays a promoting effect on the cells viability, invasiveness and motility in breast carcinoma by activating TLR/NLR signaling pathway.

Mon Feb 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: caspase recruitment domain family, member 15; caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1; NLR family, CARD domain containing 2; NOD 2; NOD-2; NOD-like receptor C2; NOD2 protein; nucleotide-binding oligomerization domain 2; nucleotide-binding oligomerization domain containing 2; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2; Nucleotide-binding oligomerization domain-containing protein 2

Gene Aliases: ACUG; BLAU; CARD15; CD; CLR16.3; IBD1; NLRC2; NOD2; NOD2B; PSORAS1

UniProt ID: (Human) Q9HC29

Entrez Gene ID: (Human) 64127

Function(s)

actin binding protein binding ATP binding enzyme binding protein kinase binding Hsp70 protein binding muramyl dipeptide binding peptidoglycan binding CARD domain binding Hsp90 protein binding non-motor microtubule binding protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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