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NOL12 Antibody (A302-732A) in IHC (P)
Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (Product # A302-732A) used at a dilution of 1:1,000 (1 µg/mL) Detection: DAB.
产品信息
A302-732A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 75 and 125 of human Nucleolar Protein 12.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
tris citrate/phosphate, pH 7-8
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, X. tropicalis and Bovine
靶标信息
Nop25 is a nucleolar protein predominantly involved in the architecture and function of the nucleolus, which is the cellular hub for ribosomal RNA (rRNA) synthesis and ribosome assembly. It is identified as a putative RNA-binding protein and plays a crucial role in rRNA transcription. Nop25 has been characterized through molecular cloning, showing its ability to bind single-stranded nucleic acids directly. Experimental studies, such as those involving mouse embryogenesis, have utilized techniques like RT-PCR to analyze Nop25's gene expression across developmental stages. Moreover, research on the protein's structure has unveiled its nuclear and nucleolar targeting sequences, vital for its localization to the nucleolus. Treatment of cells with RNase A has illustrated the dissociation of Nop25 from the nucleolus, further highlighting its RNA-binding capabilities. This protein's functional importance is underscored by its role in maintaining nucleolar architecture, as demonstrated by experiments where overexpression of Nop25's N-terminal amino acids disrupted nucleolar integrity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Nucleolar protein 12
基因别名: dJ37E16.7; NOL12; Nop25
UniProt ID: (Human) Q9UGY1
Entrez Gene ID: (Human) 79159
