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Invitrogen
NOP14 Polyclonal Antibody
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Application
FIGURE: 1 / 2
NOP14 Antibody (PA5-103985) in ICC/IF
Immunocytochemistry analysis of NOP14 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOP14 polyclonal antibody (Product # PA5-103985) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103985
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NOP14(Accession P78316), corresponding to amino acid residues M338-S388.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853316
Product Specific Information
Antibody detects endogenous levels of total NOL14.
Target Information
Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NOP14 nucleolar protein homolog; Nucleolar complex protein 14; Nucleolar protein 14; probable nucleolar complex protein 14
Gene Aliases: 2610033H07Rik; C4orf9; NOL14; NOP14; RES4-25; RES425; RGD1305605; UTP2
UniProt ID: (Human) P78316, (Mouse) Q8R3N1
Entrez Gene ID: (Human) 8602, (Mouse) 75416, (Rat) 289724
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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