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NPHP3 Antibody (PA5-97123) in WB
Western blot analysis of NPHP3 in extracts of various cell lines. Samples were incubated with NPHP3 Polyclonal antibody (Product # PA5-97123) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-97123
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NPHP3 (NP_6949723)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808925
Product Specific Information
Immunogen sequence: MGTASSLVSP AGGEVIEDTY GAGGGEACEI PVEVKPKARL LRNSFRRGAG AAAGAGPGSL PRGVGAGGLL GASFKSTGSS VPELEYAAAE YERLRKEYEI FRVSKNQELL SMGRREAKLD TENKRLRAEL; Positive Samples: 293T, Mouse liver; Cellular Location: Cell projection, cilium
Target Information
Required for normal ciliary development and function. Inhibits disheveled-1-induced canonical Wnt-signaling activity and may also play a role in the control of non-canonical Wnt signaling which regulates planar cell polarity. Probably acts as a molecular switch between different Wnt signaling pathways. Required for proper convergent extension cell movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cilia and flagella associated protein 31; Meckel syndrome, type 7; Nephrocystin-3
Gene Aliases: 3632410F03Rik; AI550417; C230078J01; CFAP31; D330020E01Rik; KIAA2000; MKS7; NPH3; NPHP3; pcy; RHPD; RHPD1; SLSN3
UniProt ID: (Human) Q7Z494, (Mouse) Q7TNH6
Entrez Gene ID: (Human) 27031, (Mouse) 74025
kidney development
heart looping
atrial septum development
lipid metabolic process
establishment or maintenance of cell polarity
determination of left/right symmetry
Wnt signaling pathway
extracellular matrix organization
lung development
regulation of cAMP metabolic process
determination of pancreatic left/right asymmetry
photoreceptor cell maintenance
maintenance of organ identity
convergent extension involved in gastrulation
cilium morphogenesis
kidney morphogenesis
determination of intestine left/right asymmetry
determination of stomach left/right asymmetry
determination of liver left/right asymmetry
ureter development
negative regulation of canonical Wnt signaling pathway
epithelial cilium movement involved in determination of left/right asymmetry
regulation of Wnt signaling pathway, planar cell polarity pathway
regulation of planar cell polarity pathway involved in neural tube closure
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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