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Alomone Labs, LTD
NPY5R Polyclonal Antibody
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Application
FIGURE: 1 / 3
NPY5R Antibody (ANR-025-200UL) in ICC/IF
Expression of neuropeptide Y5 receptor in rat DRG - Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R Antibody (#ANR-025), (1:100) followed by goat- Anti-rabbit-AlexaFluor-488 secondary Antibody . B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E. Magnification:A-C: x20E-F: x100.
Product Details
ANR-025-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R, 3rd intracellular loop
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.7 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:7958; Neuropeptide Y receptor type 5; Neuropeptide Y5 receptor; NPY-Y5 receptor; NPY5 receptor; NPY5-R; NPYY5; NPYY5-R; Y5 receptor
Gene Aliases: Npy5; NPY5-R; Npy5r; NPYR5; NPYY5-R; Y5R
UniProt ID: (Rat) Q63634, (Mouse) O70342
Entrez Gene ID: (Rat) 25340, (Mouse) 18168
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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