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NRCAM Antibody (MA5-27673) in ICC/IF
Immunofluorescent analysis of NRCAM in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NRCAM monoclonal antibody (Product # MA5-27673) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) NrCAM Antibody, D) Merged image. Magnification... View More
产品信息
MA5-27673
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N364/51
抗原
Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2735297
产品详细信息
1 µg/mL of MA5-27673 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 160kDa.
This antibody was formerly sold as clone S364-51.
靶标信息
Neuron-glia-related cell adhesion molecule (NRCAM) is a gene located on chromosome 7q31.1, encoding a protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. NRCAM is expressed in the nervous system and plays a vital role in the development and maintenance of neural connections. This adhesion molecule facilitates neuron-neuron and neuron-glia interactions, impacting processes such as axon guidance, synapse formation, and cell signaling, which are crucial for neural development and plasticity. NRCAM is involved in the modulation of signal transduction pathways that influence neuronal growth and repair, contributing to the stabilization of synaptic structures. Alterations in NRCAM expression or function have been linked to neurodevelopmental disorders, including autism spectrum disorders, and certain psychiatric conditions, reflecting its importance in maintaining neural circuit integrity. Research into NRCAM continues to explore its therapeutic potential in neurobiology and its implications in neurological and psychiatric disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ankyrin-binding cell adhesion molecule NrCAM; hBravo; mBravo; MGC138845; MGC138846; neuron-glia-CAM-related cell adhesion molecule; Neuronal cell adhesion molecule; Neuronal surface protein Bravo; Ng-CAM-related; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM; rBravo
基因别名: Bravo; C030017F07Rik; C130076O07Rik; KIAA0343; mKIAA0343; NRCAM
UniProt ID: (Human) Q92823, (Mouse) Q810U4
Entrez Gene ID: (Human) 4897, (Mouse) 319504, (Rat) 497815
