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Proteintech

NSE Polyclonal Antibody

Name: NSE Polyclonal Antibody
Brand: Proteintech
SKU: 10149-1-AP
Price: 492.0 USD
Availability: InStock

24 References

View all (95) NSE antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)

Application

NSE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

NSE Antibody (10149-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 10149-1-AP ( NSE antibody at dilution of 1:200 (under 40x lens).

NSE Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10149-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NSE Fusion Protein Ag0196 (2-300 aa encoded by BC002745)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD (2-300 aa encoded by BC002745)

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isolation, culture, and characterization of duck primary neurons.

Poultry science

Yang S,Wu J,Xian X,Chen Q

Wed Mar 01 00:00:00 UTC 2023

Neuron-Derived Exosomes Promote the Recovery of Spinal Cord Injury by Modulating Nerve Cells in the Cellular Microenvironment of the Lesion Area.

Molecular neurobiology

Xu Y,Zhu ZH,Xu X,Sun HT,Zheng HM,Zhang JL,Wang HH,Fang JW,Liu YZ,Huang LL,Song ZW,Liu JB

Tue Aug 01 00:00:00 UTC 2023

Neuron-Derived Exosomes Promote the Recovery of Spinal Cord Injury by Modulating Nerve Cells in the Cellular Microenvironment of the Lesion Area.

Molecular neurobiology

Xu Y,Zhu ZH,Xu X,Sun HT,Zheng HM,Zhang JL,Wang HH,Fang JW,Liu YZ,Huang LL,Song ZW,Liu JB

Tue Aug 01 00:00:00 UTC 2023

In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.

Materials today. Bio

Zhang D,Ren Y,He Y,Chang R,Guo S,Ma S,Guan F,Yao M

Wed Jun 01 00:00:00 UTC 2022

Inhibition of ROCK2 kinase activity improved behavioral deficits and reduced neuron damage in a DEACMP rat model.

Brain research bulletin

Xu L,Liu X,Guo C,Wang C,Zhao J,Zhang X,Li W,Zhang P,Gu R

Tue Mar 01 00:00:00 UTC 2022

Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.

International journal of biological macromolecules

Zhang D,Chang R,Ren Y,He Y,Guo S,Guan F,Yao M

Mon Oct 31 00:00:00 UTC 2022

Monosodium urate crystals regulate a unique JNK-dependent macrophage metabolic and inflammatory response.

Cell reports

Cobo I,Cheng A,Murillo-Saich J,Coras R,Torres A,Abe Y,Lana AJ,Schlachetzki J,Liu-Bryan R,Terkeltaub R,Sanchez-Lopez E,Glass CK,Guma M

Tue Mar 08 00:00:00 UTC 2022

Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.

Materials today. Bio

Wang L,Zhang D,Ren Y,Guo S,Li J,Ma S,Yao M,Guan F

Sat Jan 01 00:00:00 UTC 2022

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer.

Cell & bioscience

Wang Q,Chen J,Singh S,Xie Z,Qin F,Shi X,Cornelison R,Li H,Huang H

Sat Sep 10 00:00:00 UTC 2022

International journal of molecular sciences

Luo T,Peng J,Li Q,Zhang Y,Huang Y,Xu L,Yang G,Tan D,Zhang Q,Tan Y

Tue Nov 08 00:00:00 UTC 2022

Modeling Traumatic Brain Injury in Human Cerebral Organoids.

Cells

Ramirez S,Mukherjee A,Sepulveda S,Becerra-Calixto A,Bravo-Vasquez N,Gherardelli C,Chavez M,Soto C

Thu Oct 07 00:00:00 UTC 2021

Evaluation of cytokines and structural proteins to analyze the pathology of febrile central nervous system disease.

Legal medicine (Tokyo, Japan)

Ikeda T,Tani N,Watanabe M,Hirokawa T,Ikeda K,Morioka F,Ishikawa T

Thu Jul 01 00:00:00 UTC 2021

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.

Aging

Li F,Liu H,Zhang K,Xiao DJ,Wang C,Wang YS

Wed Jan 20 00:00:00 UTC 2021

Pan-Cancer Analysis of Glycolytic and Ketone Bodies Metabolic Genes: Implications for Response to Ketogenic Dietary Therapy.

Frontiers in oncology

Qian L,Li Y,Cao Y,Meng G,Peng J,Li H,Wang Y,Xu T,Zhang L,Sun B,Li B,Yu D

Wed Apr 27 00:00:00 UTC 2022

miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.

American journal of physiology. Gastrointestinal and liver physiology

Hong Y,Ren X,Liu W,Sun K,Chen B,Liu B,Yu X,Chen Q,Qian Q,Xie X,Jiang C

Fri Oct 01 00:00:00 UTC 2021

Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury.

STAR protocols

Ramirez S,Mukherjee A,Sepulveda SE,Gherardelli C,Becerra-Calixto A,Bravo-Vasquez N,Soto C

Fri Dec 17 00:00:00 UTC 2021

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.

Cell cycle (Georgetown, Tex.)

Wang Z,Wang X,Bi M,Hu X,Wang Q,Liang H,Liu D

Mon Feb 01 00:00:00 UTC 2021

Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy.

Neuroscience letters

Cao H,Zhu X,Zhang J,Xu M,Ge L,Zhang C

Mon Mar 23 00:00:00 UTC 2020

Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy.

Neuroscience letters

Cao H,Zhu X,Zhang J,Xu M,Ge L,Zhang C

Mon Mar 23 00:00:00 UTC 2020

Intranasal administration of Cytoglobin modifies human umbilical cord‑derived mesenchymal stem cells and improves hypoxic‑ischemia brain damage in neonatal rats by modulating p38 MAPK signaling‑mediated apoptosis.

Molecular medicine reports

Yang H,Tian S,Xie L,Chen Y,Ma L

Thu Oct 01 00:00:00 UTC 2020

Identification of chimeric RNAs in human infant brains and their implications in neural differentiation.

The international journal of biochemistry & cell biology

Tang Y,Ma S,Wang X,Xing Q,Huang T,Liu H,Li Q,Zhang Y,Zhang K,Yao M,Yang GL,Li H,Zang X,Yang B,Guan F

Sat Jun 01 00:00:00 UTC 2019

A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in rats.

Biomaterials science

Yao M,Gao F,Xu R,Zhang J,Chen Y,Guan F

Tue Sep 24 00:00:00 UTC 2019

Nell-1 promotes the neural-like differentiation of dental pulp cells.

Biochemical and biophysical research communications

Han Q,Wang Q,Wu J,Li M,Fang Y,Zhu H,Wang X

Tue May 28 00:00:00 UTC 2019

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Journal of molecular histology

Owusu L,Xu C,Chen H,Liu G,Zhang G,Zhang J,Tang Z,Sun Z,Yi X

Wed Aug 01 00:00:00 UTC 2018

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Journal of molecular histology

Owusu L,Xu C,Chen H,Liu G,Zhang G,Zhang J,Tang Z,Sun Z,Yi X

Wed Aug 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Eno; ENOG; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; gamma enolase; Gamma-enolase; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuron-specific enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; NSE

Gene Aliases: AI837106; D6Ertd375e; Eno-2; ENO2; HEL-S-279; NSE; RNEN3

UniProt ID: (Human) P09104, (Mouse) P17183, (Rat) P07323

Entrez Gene ID: (Human) 2026, (Mouse) 13807, (Rat) 24334

Function(s)

magnesium ion binding phosphopyruvate hydratase activity protein binding protein homodimerization activity protein heterodimerization activity lyase

Process(es)

gluconeogenesis canonical glycolysis glycolytic process response to organic cyclic compound response to estradiol response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NSE Polyclonal Antibody

NSE Antibody (10149-1-AP) in IHC (P)

Product Details

10149-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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