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NSF Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

OSN00015G-500UG

Applications

Tested Dilution

Publications

Western Blot (WB)

10-50 µg/mL

Immunohistochemistry (IHC)

10-50 µg/mL

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from n-terminal of human NSF conjugated to an immunogenic carrier protein was used as the antigen

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in mouse and rat.

Specificity of this antibody: NSF.

Target Information

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.

Journal of proteome research

Ballif BA,Carey GR,Sunyaev SR,Gygi SP

Tue Jan 01 00:00:00 EST 2008

The transcriptional landscape of the mammalian genome.

Science (New York, N.Y.)

Carninci P,Kasukawa T,Katayama S,Gough J,Frith MC,Maeda N,Oyama R,Ravasi T,Lenhard B,Wells C,Kodzius R,Shimokawa K,Bajic VB,Brenner SE,Batalov S,Forrest AR,Zavolan M,Davis MJ,Wilming LG,Aidinis V,Allen JE,Ambesi-Impiombato A,Apweiler R,Aturaliya RN,Bailey TL,Bansal M,Baxter L,Beisel KW,Bersano T,Bono H,Chalk AM,Chiu KP,Choudhary V,Christoffels A,Clutterbuck DR,Crowe ML,Dalla E,Dalrymple BP,de Bono B,Della Gatta G,di Bernardo D,Down T,Engstrom P,Fagiolini M,Faulkner G,Fletcher CF,Fukushima T,Furuno M,Futaki S,Gariboldi M,Georgii-Hemming P,Gingeras TR,Gojobori T,Green RE,Gustincich S,Harbers M,Hayashi Y,Hensch TK,Hirokawa N,Hill D,Huminiecki L,Iacono M,Ikeo K,Iwama A,Ishikawa T,Jakt M,Kanapin A,Katoh M,Kawasawa Y,Kelso J,Kitamura H,Kitano H,Kollias G,Krishnan SP,Kruger A,Kummerfeld SK,Kurochkin IV,Lareau LF,Lazarevic D,Lipovich L,Liu J,Liuni S,McWilliam S,Madan Babu M,Madera M,Marchionni L,Matsuda H,Matsuzawa S,Miki H,Mignone F,Miyake S,Morris K,Mottagui-Tabar S,Mulder N,Nakano N,Nakauchi H,Ng P,Nilsson R,Nishiguchi S,Nishikawa S,Nori F,Ohara O,Okazaki Y,Orlando V,Pang KC,Pavan WJ,Pavesi G,Pesole G,Petrovsky N,Piazza S,Reed J,Reid JF,Ring BZ,Ringwald M,Rost B,Ruan Y,Salzberg SL,Sandelin A,Schneider C,Schönbach C,Sekiguchi K,Semple CA,Seno S,Sessa L,Sheng Y,Shibata Y,Shimada H,Shimada K,Silva D,Sinclair B,Sperling S,Stupka E,Sugiura K,Sultana R,Takenaka Y,Taki K,Tammoja K,Tan SL,Tang S,Taylor MS,Tegner J,Teichmann SA,Ueda HR,van Nimwegen E,Verardo R,Wei CL,Yagi K,Yamanishi H,Zabarovsky E,Zhu S,Zimmer A,Hide W,Bult C,Grimmond SM,Teasdale RD,Liu ET,Brusic V,Quackenbush J,Wahlestedt C,Mattick JS,Hume DA,Kai C,Sasaki D,Tomaru Y,Fukuda S,Kanamori-Katayama M,Suzuki M,Aoki J,Arakawa T,Iida J,Imamura K,Itoh M,Kato T,Kawaji H,Kawagashira N,Kawashima T,Kojima M,Kondo S,Konno H,Nakano K,Ninomiya N,Nishio T,Okada M,Plessy C,Shibata K,Shiraki T,Suzuki S,Tagami M,Waki K,Watahiki A,Okamura-Oho Y,Suzuki H,Kawai J,Hayashizaki Y

Fri Sep 02 00:00:00 EDT 2005

Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast.

FEBS letters

Périer F,Coulter KL,Liang H,Radeke CM,Gaber RF,Vandenberg CA

Mon Sep 05 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: N-ethylmaleimide sensitive factor; N-ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Protein SKD2; Suppressor of K(+) transport growth defect 2; Vesicle-fusing ATPase; Vesicular-fusion protein NSF

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Gene Aliases: AI316878; AU020090; AU067812; Erg1; NSF; SKD2

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UniProt ID: (Human) P46459, (Mouse) P46460, (Rat) Q9QUL6

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Entrez Gene ID: (Human) 4905, (Mouse) 18195, (Rat) 60355

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Function(s)

protein binding ATP binding ATPase activity syntaxin-1 binding Rab GTPase binding protein kinase binding PDZ domain binding protein complex binding ATPase activity, coupled metal ion binding nucleotide binding protein C-terminus binding hydrolase activity syntaxin binding D1 dopamine receptor binding ATP-dependent protein binding membrane traffic protein

Process(es)

positive regulation of receptor recycling potassium ion transport intracellular protein transport exocytosis ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER intra-Golgi vesicle-mediated transport vesicle-mediated transport regulation of exocytosis Golgi to plasma membrane protein transport plasma membrane fusion positive regulation of protein catabolic process COPII vesicle coating Golgi vesicle docking transport metabolic process protein transport vesicle fusion neurotransmitter secretion membrane fusion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NSF Polyclonal Antibody

Product Details

OSN00015G-500UG

Western Blot (WB)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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