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Product Details
PA5-121024
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
Conjugate
Form
Liquid
Concentration
1.49 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914596
Product Specific Information
Positive test controls include: 293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney. The target is usually found in the following locations: Chromosome, Golgi apparatus, Golgi stack, Nucleus.
Immunogen sequence: MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG EKRQHSSQDV HVVLK
Target Information
p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; p97 cofactor p47; RP4-776F14.2; SHP1 homolog; UBX domain-containing protein 2C; XY body-associated protein XY40
Gene Aliases: dJ776F14.1; Munc-18c; NSFL1C; P47; Stxbp3a; UBX1; UBXD10; UBXN2C; XY40
UniProt ID: (Human) Q9UNZ2, (Rat) O35987, (Mouse) Q9CZ44
Entrez Gene ID: (Human) 55968, (Rat) 83809, (Mouse) 386649
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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