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Invitrogen
NUMB Monoclonal Antibody (S.925.4)
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Application
FIGURE: 1 / 7
NUMB Antibody (MA5-14897) in ICC/IF
Immunofluorescence analysis of NUMB was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NUMB Monoclonal Antibody (S.925.4) (Product # MA5-14897) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
Product Details
MA5-14897
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
S.925.4
Immunogen
Synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein
Conjugate
Unconjugated
Form
Liquid
Concentration
88 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985378
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: h-Numb; m-numb; mnb; numb homolog; Protein numb homolog; Protein S171
Gene Aliases: c14_5527; C14orf41; NUMB; S171
UniProt ID: (Human) P49757
Entrez Gene ID: (Human) 8650
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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