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Invitrogen

NUP107 Monoclonal Antibody (39C7)

Name: NUP107 Monoclonal Antibody (39C7)
Brand: Invitrogen
SKU: MA110031
Price: 504.0 USD
Availability: InStock

6 Published Figures

4 References

View all (15) NUP107 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (6)

Application

NUP107 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

NUP107 Antibody (MA1-10031) in ICC/IF

Immunofluorescence analysis of NUP107 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with NUP107 Monoclonal Antibody (39C7) (Product # MA1-10031) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More

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Product Details

MA1-10031

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Fruit fly, Horse, Human, Mouse, Pig, Rat, Yeast

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

39C7

Immunogen

Yeast nuclear preparation.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2154446

Product Specific Information

For immunofluorescence on yeast cells, try MCA-39C7 diluted 1:100 to 1:500. For immunofluorescence on mammalian cells try at 1:50 to 1:100.

Target Information

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleoporin93 limits Yap activity to prevent endothelial cell senescence.

Aging cell

Nguyen TD,Rao MK,Dhyani SP,Banks JM,Winek MA,Michalkiewicz J,Lee MY

Published figure using NUP107 monoclonal antibody (Product # MA1-10031) in Western Blot

Mon Apr 01 00:00:00 UTC 2024

Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation.

Scientific reports

Gonzalez Pisfil M,Nadelson I,Bergner B,Rottmeier S,Thomae AW,Dietzel S

MA1-10031 was used in Immunocytochemistry-immunoflourescence to provide evidence that eight color FLIM-STED with a single depletion laser would be possible if suitable fluorochromes were identified and we confirm that a fluorochrome may have different lifetimes depending on the molecules to which it is coupled.

Thu Aug 18 00:00:00 UTC 2022

Nuclear Pore Complexes Cluster in Dysmorphic Nuclei of Normal and Progeria Cells during Replicative Senescence.

Cells

Röhrl JM,Arnold R,Djabali K

Published figure using NUP107 monoclonal antibody (Product # MA1-10031) in Immunocytochemistry

Thu Jan 14 00:00:00 UTC 2021

G₄C₂ Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Neuron

Coyne AN,Zaepfel BL,Hayes L,Fitchman B,Salzberg Y,Luo EC,Bowen K,Trost H,Aigner S,Rigo F,Yeo GW,Harel A,Svendsen CN,Sareen D,Rothstein JD

MA1-10031 was used in Immunocytochemistry to suggest that the expression of expanded C9orf72 ALS/FTD repeat RNA alone affects nuclear POM121 expression in the initiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately downstream neuronal survival.

Wed Sep 23 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 107 kDa nucleoporin; CG6743-PA; nuclear pore complex; Nuclear pore complex protein Nup107; Nuclear pore complex protein Nup107-like protein; Nuclear pore protein nup107; nucleoporin 107; nucleoporin 107kDa; Nucleoporin Nup107; Nup107-PA; Nup170; p105

Gene Aliases: AW541137; BcDNA:LD18761; C76801; CG6743; Dmel\CG6743; Dmel_CG6743; dmNup107; NPHS11; Nup; Nup 107; NUP107; Nup170; NUP84; SPBC428.01c; SPBC582.11c

UniProt ID: (Human) P57740, (Fruit fly) Q9V466, (Mouse) Q8BH74, (Fission yeast) Q10331

Entrez Gene ID: (Human) 57122, (Horse) 100059287, (Chicken) 101751988, (Pig) 100516909, (Fruit fly) 34481, (Rat) 116555, (Mouse) 103468, (Fission yeast) 3361255

Function(s)

nucleocytoplasmic transporter activity protein binding structural constituent of nuclear pore chromatin binding porin activity transporter

Process(es)

posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery regulation of transcription, DNA-templated mRNA export from nucleus tRNA export from nucleus protein import into nucleus sister chromatid cohesion mitotic nuclear envelope disassembly regulation of glucose transport viral process protein sumoylation viral transcription gene silencing by RNA nuclear pore complex assembly intracellular transport of virus regulation of cellular response to heat double-strand break repair via homologous recombination meiosis I cytokinesis male meiosis cytokinesis female gonad development eggshell formation regulation of nucleocytoplasmic transport spermatocyte division positive regulation of protein localization to nucleus protein transport transmembrane transport transport mRNA transport rRNA export from nucleus poly(A)+ mRNA export from nucleus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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