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Nanog Antibody (A300-397A) in WB
Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for IP at 6 µg per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody A300-398A. For blotting immunoprecipitated Nanog, A300-397A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds... View More
Product Details
A300-397A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 1 and 50 of mouse Nanog homeobox.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; Homeobox protein NANOG; Homeobox transcription factor Nanog
Gene Aliases: 2410002E02Rik; Ecat4; ENK; Nanog
UniProt ID: (Mouse) Q80Z64
Entrez Gene ID: (Mouse) 71950
negative regulation of transcription from RNA polymerase II promoter
mesodermal cell fate commitment
endodermal cell fate specification
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell proliferation
positive regulation of cell proliferation
embryo development
response to organic substance
negative regulation of cell fate commitment
regulation of gene expression
stem cell division
stem cell population maintenance
negative regulation of BMP signaling pathway
response to retinoic acid
somatic stem cell population maintenance
negative regulation of endodermal cell fate specification
regulation of cell differentiation
negative regulation of cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
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